Influence of sire genotype and cattle breed gene pool on sperm cryosurvival
A. I. Zheltikov,
T. V. Konovalova,
N. M. Kostomakhin,
O. S. Korotkevich,
O. I. Sebezhko,
V. L. Petukhov,
M. V. Strizhkova Abstract
This study characterized sires of Red-and-White, Black-and-White, and Red Steppe cattle breeds at JSC Barnaul Bull Stud Enterprise (Altai Krai, Russia) in terms of sperm production quality and resistance to freezing. A total of 50,388 ejaculates were examined before cryopreservation, including 38,333 that were frozen. The influence of individual sires was assessed on 1,716 ejaculates. Sires of the Black-and-White breed had the highest rate (31%) of sperm culling before cryopreservation. This rate was 9–18.2 percentage points higher than sires of the other two breeds (p < 0.001). The difference in frequency of ejaculates culled after thawing was significantly lower between the breeds. However, a significant difference existed between sires of the Red Steppe breed and the other two breeds (p < 0.001), with the Red Steppe breed having 1.6 and 2.0 percentage points lower culling rates. More significant differences in the frequency of ejaculates culled before cryopreservation were observed between individual sires within the Red Steppe breed than between breeds, with a maximum difference of 31.9%. A strong negative rank correlation (r = –0.90) was found between ejaculate volume and culling before freezing. There was no significant difference between the sires of Red Steppe breed in the frequency of culling ejaculates after thawing. Thus, this study demonstrates the influence of sire genotype and breed gene pool on sperm cryosurvival.
About the Authors
A. I. Zheltikov
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Alexander I. Zheltikov, DSc (Ag), Professor, Professor at the Department of Veterinary Genetics and Biotechnology
160 Dobrolyubova St., Novosibirsk, 630039
T. V. Konovalova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Tatyana V. Konovalova, Senior Lecturer at the Department of Veterinary Genetics and Biotechnology
160 Dobrolyubova St., Novosibirsk, 630039
N. M. Kostomakhin
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Nikolay M. Kostomakhin, DSc (Bio), Professor, Professor at the Department of Dairy and Beef Cattle Breeding
49 Timiryazevskaya St., Moscow, 127550
O. S. Korotkevich
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Olga S. Korotkevich, DSc (Bio), Professor, Professor at the Department of Veterinary Genetics and Biotechnology
160 Dobrolyubova St., Novosibirsk, 630039
O. I. Sebezhko
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Olga I. Sebezhko, CSc (Bio), Associate Professor, Associate Professor at the Department of Veterinary Genetics and Biotechnology
160 Dobrolyubova St., Novosibirsk, 630039
V. L. Petukhov
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Valeriy L. Petukhov, DSc (Bio), Professor, Professor at the Department of Veterinary Genetics and Biotechnology
160 Dobrolyubova St., Novosibirsk, 630039
M. V. Strizhkova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Maria V. Strizhkova, CSc (Bio), Associate Professor at the Department of Therapy, Surgery and Obstetrics
160 Dobrolyubova St., Novosibirsk, 630039
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Review
For citations:
Zheltikov A.I., Konovalova T.V., Kostomakhin N.M., Korotkevich O.S., Sebezhko O.I., Petukhov V.L., Strizhkova M.V. Influence of sire genotype and cattle breed gene pool on sperm cryosurvival. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2025;(3):139-150. (In Russ.)
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