Конкурентная аллель-специфичная ПЦР (KASP): особенности, интерпретация результатов

Использование конкурентной аллель-специфичной ПЦР становится популярным методом для массового генотипирования сельскохозяйственных культур. Разработка маркеров на SNP в полиплоидных организмах требует дополнительной проверки и тестирования при разработке праймеров для дальнейшего использования. Предлагается алгоритм проверки и интерпретации работоспособности KASP-маркеров на пшенице с помощью существующих геномных сборок мягкой пшеницы. С помощью описанного алгоритма было проанализировано 6 KASP-маркеров на гены VIVIPAROUS-1, MOTHER OF FT AND TFL1, TGW6-A1, PSY-A1, Dreb-B1 и локус устойчивости к листовой ржавчине Lr14a. Наиболее перспективными KASP-маркерами по результатам биоинформатического анализа и ПЦР оказались маркеры на гены VIVIPAROUS-1, MOTHER OF FT AND TFL1.

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