No 6 (2020)
AGRONOMY, CROP PRODUCTION, PLANT PROTECTION
LIVESTOCK BREEDING, BIOLOGY AND VETERINARY MEDICINE
16-25 181
Abstract
The need to improve the quality and accuracy of measurements of the composition and properties of milk and dairy products leads to the wider use of modern analysis methods, including methods of molecular genetic research. The use of the PCR analysis method to identify the species composition of dairy products is the most promising. The paper considers the possibility of developing a measurement technique for assessing the composition and properties of milk and dairy products using molecular genetic methods of analysis (PCR, PCR-RFLP, and sequencing). Evaluation of the sensitivity of the method and its metrological characteristics helped determine the most suitable research method.
26-33 104
Abstract
Modern aquaculture is rapidly developing. It includes food fish farming and decorative fish breeding. Like in any fast-growing livestock industry, there may be problems related to the health offish. Carp is the main object in Russian fish farming, representing the basic type of unpretentious, omnivorous and fast-growing pond polyculture. Tilapia is one of the most preferred species of global aquaculture, due to its hardiness, rapid weight gain and ability to tolerate high planting density. Discus is the most popular of the ornamental fish species; it features a large number of color variations, interesting behavior and is cost-effective in breeding. The authors carried out comparative physiological assessment of bony fish of the cyprinid and cichlid families. Hematological parameters were determined - erythrocyte and leukocyte formulas (by means of differential analysis techniques); biochemical parameters of blood serum (with the analyzer), the average cytochemical coefficient of lysosomal cationic protein content in fish blood neutrophils. The research results showed specific features in the intensity of erythro- and leukopoiesis, the proportion of granular cells of the myeloid series. It was revealed that the peculiarity of discus is the presence of blast forms of white blood cells in the blood: myeloblasts, promyelocytes, and myelocytes. In the blood of tilapia, in contrast to discus and carp, there is a high content of granulocytes: neutrophils, eosinophils and basophils of monocytes and lymphocytes in the leukogram. Differences between species were noted based on the results of the lysosomal cation test. The difference in a number of biochemical parameters can also be seen: in tilapia as compared with other species - in the content of albumins, alkaline phosphatase and ALT, in discus - in the activity of lactate and KK enzymes.
34-48 138
Abstract
The authors studied the growth characteristics (morphophysiological indicators) of broilers ’ digestive organs when using Pharmatan in the diet at doses of 0.025; 0.05 and 0.075%. For the study, three medium-weight broiler chickens were selected - on a daily basis for the first three days, then on a weekly basis. The mass of the stomach, individual intestines, the mass of internal organs, and the coefficient of growth rate were determined according to Brody. To study the relative growth rate, the simple allometry formula y = axb was used. It has been established that the most intensive growth of the digestive organs is observed in the first week of broiler life and consists in the stimulated growth of the intestine, especially the cecum. Later on, the growth of the digestive organs in birds of the control group increases and becomes equal to or higher than in broilers of the experimental group. In general, for 42 days of the experiment, the growth rate of the organs of the gastrointestinal tract is practically the same in the birds of the experimental and control groups. The parameters of the regression equations indicate significant differences in the growth of individual organs of the digestive tube during the individual development of broilers. In the experimental groups of broilers, almost all intestinal organs grow quite intensively (b = 1.325-1.783) during the first week of raising, with relatively low values of determination coefficients (cecum - R2 = 0.355; jejunum - R2 = 0.745). High morphological values of 7-day-old birds treated with Pharmatan form the basis for growing broilers with a higher live weight.
49-61 105
Abstract
The paper provides information about the growth and development of somatic structures of two-year-old carp during the fattening period. In variant I, K-III compound feed was used (low-carb-monodiet), in variant II - Carp-38/12 (high-carb-monodiet), in variant III - K-III and Carp-38/12 (in different auto-feeders). The highest proportion of axial somatic structures was observed in fish of variant III (fish feeding on a selective basis) (P < 0.05). White muscles are also better developed in fish that eat complex feed (variant III). Compound feed Carp 38/12 does not increase the mass of white muscles (P < 0.05). The proportion of red muscles in fish that eat mixed feed with a low fat content (3.5%) is 45.5% lower than in variants II and III. The use of feed with different energy values and their combination in one pond affects the growth and development of the structure that determines the growth offish species - the axial skeletal muscles. At the same time, the two-year-old fish species that received the most energy-intensive diet were the most well-fed. Two-year-old carp that received feed with different energy content and complex composition grow differently. This is primarily due to the growth of muscles, which is evidenced by allometric coefficients. The highest values of this parameter featuring positive allometry, were observed in two-year-old carp that received two compound feeds from different feeders (white muscles b = 1.106; red muscles b = 1.499; all muscles b = 1.125). The bionic method of feeding fish in combination with feeds of different energy values provides for strengthened growth of one of the most important body structures - the somatic muscle system. It is not only a biological component of the body that ensures the welfare of animals, but also a high-quality food source.
ISSN 0021-342X (Print)