Integrated approach for accelerated breeding of spring rapeseed F1-hybrids based on CMS

The breeding of F1-hybrids of spring rapeseed is the most effective approach to solving the problem of increasing rapeseed yield, as well as provides a more effective return on investment in breeding. Accelerated breeding of heterosis hybrids is provided by a complex of modern biotechnological and molecular-genetic methods. Cytoplasmic male sterility (CMS) is used to control hybridization in the industrial production of F1-hybrid seeds and for additional copyright protection. Molecular-genetic markers allow rapid determination of the type of CMC and differentiation between the lines of fertility restorers and sterility maintainers. By combining double haploid production and indoor planting for the development of maternal lines, the intensity of the breeding process can be increased by 2–3 times.

In this research, we have genotyped a germplasm collection of foreign spring rape using the molecular marker ORF138 for the Ogura cytoplasm and divided the collection into two groups according to the presence or absence of the marker. Subsequently, doubled haploid lines (DHL) produced from a sample with Ogura cytoplasm were used as fertility restorer lines, and DHL from hybrids with a non-Ogura cytoplasm were used to develop female male sterile lines based on Ogura-CMC.

The doubled haploid lines were evaluated in the field for the main economically valuable traits, and 30 fertility restorer lines and four male-sterile lines were hybridized to assess their combining ability. As a result, four DH lines J30, J26, J24 and Ki1ms were selected for future breeding of heterosis hybrids basedon Ogura-CMC and five promising spring rapeseed hybrids were identified according to the complex of traits and properties.

1. Anisimova I.N., Dubovskaya A.G. CMS systems in rapeseed and their use in the breeding of domestic hybrids. Proceedings on applied botany, genetics and breeding. 2020;181(3):171–180. (In Russ.) https://doi.org/10.30901/2227-8834-2020-3-171-180

2. Bochkaryova E.B., Gorlova L.A., Serdyuk V.V., Strelnikov E.A. Breeding value of dihaploid lines of spring rape (Brassica napus L.). Oil Crops. 2019;4 (180):18–22. (In Russ.) https://doi.org/10.25230/2412-608X-2019-4-180-18-22

3. Vishnyakova A.V., Aleksandrova A.A., Monakhos S.G. Factors of direct germination of microspore derived embryos of Brassica napus L. Izvestiya of Timiryazev Agricultural Academy (TAA). 2022;1(6):43–53. (In Russ.) https://doi.org/10.26897/0021-342X-2022-6-43-53

4. Goncharov S.V., Dolgikh L.A. Reformatting the CIS rapeseed seed market over the last 30 years. Aktual’nye problemy agronomii sovremennoy Rossii i puti ikh resheniya. 2018:113–120. (In Russ.)

5. Goncharov S.V., Gorlova L.A. Oil crops: new challenges and trends in their development. Oil Crops. 2018;2(174):96–100. (In Russ.) https://doi.org/10.25230/2412-608KH-2018-2-174-96-100

6. Golovachev V.I. Methods of varietal testing of agricultural crops. Moscow: Kalininskaya oblastnaya tipografiya upravleniya izdatel’stv tipografii i knizhnoy torgovli Kalininskogo oblispolkoma, 1983:184. (In Russ.)

7. Gorlova L.A., Bochkaryova E.B., Strelnikov E.A., Serdyuk V.V. The use of classical and modern methods in rapeseed (Brassica napus) breeding at VNIIMK. Proceedings on Applied Botany, Genetics and Breeding. 2020;180(4):126–131. (In Russ.) https://doi.org/10.30901/2227-8834-2019-4-126-131

8. Domblides E.A., Domblides A.S., Zayachkovskaya T.V., Bondareva L.L. Identification of cytoplasm types in accessions of the family Brassicaceae (Brassicaceae Burnett) with DNA markers. Vavilov Journal of Genetics and Breeding. 2015;19(5):529–537. (In Russ.) https://doi.org/10.18699/VJ15.069

9. Karpachev V.V., Pastukhov I.O. Estimation of combining ability of androcline lines of spring rape in diallel crosses. Oil Crops. 2017;1(169):40–42. (In Russ.)

10. RASRAPS: Association of Rapeseed Producers and Processors. (In Russ.) URL: https://rosraps.ru/ru/ (Access date: 12.10.2023)

11. Savchenko V.K. Multipurpose method of quantitative evaluation of combining ability in selection for heterosis. Russian Journal of Genetics. 1978;5:793. (In Russ.)

12. Brandt S.A. et al. Seeding rate, fertilizer level and disease management effects on hybrid versus open pollinated canola (Brassica napus L.). Canadian Journal of Plant Science. 2007;87(2):255–266. https://doi.org/10.4141/P05-223

13. Corral-Martínez P., Camacho-Fernandez C., Mir R., Seguı´-Simarro J.M. Doubled haploid production in high-and low-response genotypes of rapeseed (Brassica napus) through isolated microspore culture. Doubled haploid technology. Humana, New York, NY, 2021:129–144.

14. Grelon M., Budar F., Bonhomme S., Pelletier G. Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids. Molecular and General Genetics MGG. 1994;243:540–547.

15. Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic acids research. 1980;8(19):4321–4326.

16. Singh S., Bhatia R., Kumar R., Behera T.K., Kumari K., Pramanik A., Ghemeray H., Sharma K., Bhattacharya R.C., Dey S.S. Elucidating mitochondrial DNA markers of Ogura-based CMS lines in Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cytonuclear interactions. Frontiers in Plant Science. 2021;12:631489. https://doi.org/10.3389/fpls.2021.631489

17. Rahman M., de Jiménez M.M. Behind the scenes of microspore-based double haploid development in Brassica napus: A review. J. Plant Sci. Mol. Breed. 2016;5(1). https://doi.org/10.7243/2050-2389-5-1

18. Wolko J., Dobrzycka A., Bocianowski J., Bartkowiak-Broda I. Estimation of heterosis for yield-related traits for single cross and three-way cross hybrids of oilseed rape (Brassica napus L.). Euphytica. 2019;215(10):156. https://doi.org/10.1007/s10681-019-2482-6

19. Xing N., Fan C., Zhou Y. Parental selection of hybrid breeding based on maternal and paternal inheritance of traits in rapeseed (Brassica napus L.). PLoS One. 2014; 9(7): e103165. https://doi.org/10.1371/journal.pone.0103165