Cow rumen microbiome and milk productivity with the Profort enzyme-probiotic feed additive

The transition of dairy farming to an industrial basis contributes to an increase in the productive indicators of cattle, including due to the transition to a uniform year-round feeding. From the physiological point of view, a uniform feeding system contributes to the preservation of the species and quantitative composition of the microflora, maintaining its high enzymatic activity. With year-round uniform feeding, it is particularly important to provide the animal’s

body with biologically adequate nutrition, which depends not only on the quantity but also on the quality of the feed. The use of additives in the feed ration, including those based on probiotic preparations, helps to improve digestive processes, normalize the balance of microflora, metabolism and increase animal productivity. The aim of the research was to analyze the rumen microbiome and milk productivity of cows using the enzyme-probiotic feed additive Profort under conditions of year-round uniform feeding. The studies were conducted in lactating Holstein cows. The cows of the experimental group were fed 30.0 g of the probiotic three times during 15 days, with a 15-day interval. In the experimental group, at the family level, the taxa of cellulolytic bacteria Prevotellaceae, Oscillospiraceae, SR1. showed the most significant reliable differences The maximum total content of beneficial cellulolytic bacteria after the use of the enzymeprobiotic preparation was found in the rumen content of the cows of the experimental group. It was 75.34 ± 2.0%. The minimum content (74.89 ± 1.2%) was found in the control group. The content of lactate-utilizing bacteria, which ferment lactic acid in the rumen to volatile fatty acids, increased to an average of 1.14 ± 0.5% in the cows of the experimental group. The average daily milk yield increased from 4.7 to 6.1%, and the mass fraction of fat and protein in milk increased by 1.05–1.75% and 0.64–1.29%, respectively, depending on the feeding period.

1. Denisov, N. I. Kormlenie vy`sokoproduktivny`x korov. - Moskva: Rossel`xozizdat, 1982. - 121 s.

2. Romanenko, L.V. Polnocenny`e kormovy`e smesi dlya korov vy`sokoj i rekordnoj produktivnosti/ L.V. Romanenko, V.I. Volgin, Z.L. Fedorova, E.A. Korochkina // Nauchnoe obozrenie. Biologicheskie nauki. -2016. – № 1. -S. 90-95.

3. Xaritonov, E. L. Sovremenny`e problemy` pri organizacii normirovaniya pitaniya vy`sokoproduktivnogo molochnogo skota // Molochnoe i myasnoe skotovodstvo. - 2010. - № 4. - S. 16-18.

4. Ispol`zovanie pitatel`ny`x veshhestv zhvachny`mi zhivotny`mi / B. Piatkovskij, P. Kauffol`d, Yu. Fojgt i dr. ; Per. s nem. d. b. n. N.S. Gel`man ; Pod red. k. s.-x. n. A.M. Xolmanova. - Moskva: Kolos, 1978. - 424 s.

5. Kuryatova, E. V. Profilaktika acidoza korov probioticheskim preparatom i ego vliyanie na molochnuyu produktivnost`/ E. V. Kuryatova, O. N. Tyukavkina, O. V. Gruzdova // Dal`nevostochny`j agrarny`j vestnik. - 2021. - № 3 (59). - S. 44-54.

6. Bogolyubova, N.V., Bioximicheskij status organizma molochny`x korov i molodnyaka krupnogo rogatogo skota s ispol`zovaniem v pitanii e`nergeticheskix i fitobioticheskix komponentov / N.V.Bogolyubova, R.A. Ry`kov // Ucheny`e zapiski Kazanskoj gosudarstvennoj akademii veterinarnoj mediciny` im. N.E`.Baumana. – 2019. - T.239 (III)- C.44-51.

7. Produktivnost` korov pri razlichny`x variantax odnotipnogo kormleniya //Truxachev V.I., Zly`dnev N.Z., Dridiger V.K. i dr./nauchno-prakticheskie rekomendacii, Stavropol`skij gos. agrarny`j universitet.-Stavropol`: AGRUS,2009.-54s.

8. Reynolds, C. K. Net metabolism of volatile fatty acids, d-β-hydroxybutyrate, nonesterified fatty acids, and blood gases by portal-drained viscera and liver of lactating Holstein cows/ C. K. Reynolds, G. B. Huntington, H. F. Tyrrell, P. J. Reynolds, // Journal of Dairy Science. - 1988. - Vol. 71, No. 9. - P. 2395-2405. doi: 10.3168/jds.S0022-0302(88)79824-0.

9. Laptev G.Yu. Mikrobiom sel`skoxozyajstvenny`x zhivotny`x: svyaz` so zdorov`em i produktivnost`yu/Laptev G.Yu., Novikova N.I., Jy`ldy`ry`m E.A. i dr.-SPb: Prospekt Nauki, 2020.-336s.

10. Clemmons, B. A. Rumen Bacteria and Serum Metabolites Predictive of Feed Efficiency Phenotypes in Beef Cattle/ B. A. Clemmons, C. Martino, J. B. Powers, S. R. Campagna, B. H. Voy, D. R. Donohoe, J. Gaffney, M. M. Embree, P. R. Myer, // Sci Rep. - 2019. - Vol. 9, No. 1. - P. 19265.

11. Produktivnost` krupnogo rogatogo skota pri obogashhenii racionov probioticheskim preparatom / R. V. Nekrasov, M. G. Chabaev, A. A. Zelenchenkova [i dr.] // Molochnoe i myasnoe skotovodstvo. – 2016. – № 7. – S. 19-22. – EDN XQSWUZ.

12. Janssen, P. H. Structure of the archaeal community of the rumen/ P. H. Janssen, M. Kirs// Applied and Environmental Microbiology. - 2008. - Vol. 74, No. 12. - P. 3619-3625. doi: 10.1128/AEM.02812-07.

13. Aschenbach, J. R., Penner, G. B., Stumpff, F., & Gäbel, G. Ruminant nutrition symposium: Role of fermentation acid absorption in the regulation of ruminal pH/ J. R. Aschenbach, G. B. Penner, F. Stumpff, G. Gäbel, // Journal of Animal Science. - 2011. - Vol. 89, No. 4. - P. 1092–1107. doi:10.2527/jas.2010-3301

14. Qumar M., Evidence of in vivo absorption of lactate and modulation of short chain fatty acid absorption from the reticulorumen of non-lactating cattle fed high concentrate diets/ M. Qumar, R.Khiaosa-ard, F. Pourazad, S.Wetzels, , F. Klevenhusen, W. Kandler, J. Aschenbach, Q. Zebeli, // PLoS ONE. - 2016. - Vol. 11, No. 10. - P. e0164192. doi: 10.1371/journal.pone.0164192

15. Reuben, R. C., Elghandour, M. M. M. Y., Alqaisi, O., Cone, J. W., Márquez, O., Salem, A. Z. M. Influence of microbial probiotics on ruminant health and nutrition: sources, mode of action and implications // J Sci Food Agric. - 2022. - Vol. 102, No. 4. - P. 1319-1340. doi: 10.1002/jsfa.11643.

16. Normy` i raciony` kormleniya sel`skoxozyajstvenny`x zhivotny`x. Spravochnoe posobie. 3-e izdanie, pererabotannoe i dopolnennoe. / Pod red. A. P. Kalashnikova, V. I. Fisinina, V. V. Shheglova, N. I.Klejmenova. - Moskva. 2003. - 456 s.