Problem of reducing the quality of hemp seeds and ways to solve it
https://doi.org/10.26897/0021-342X-2024-5-58-67
Abstract
The article presents the results of studies on the influence of damage during combine harvesting and post-harvest treatment on the sowing properties of hemp seeds. It is noted that at combine threshing on the background of increase of mechanical damages in the form of macrotraumas (35%) and microtraumas (22%) there is a decrease in laboratory germination of hemp seeds up to 68%. The main share of macrotraumas is caused by crushing – 8.5%; removal of husk – 10%, as well as cracks in the area of the cotyledon and embryo – 16.5%. Desiccation of hemp crops at combine threshing and subsequent post-harvest treatment reduces macrodamage of seeds by 17% and microdamage by 9%. Indicators of germination energy and seed germination are comparable with similar data from manual threshing and amount to 94% and 95%, respectively. Microbiological analysis of hemp seeds showed significant differences in the quantitative and group composition of bacterial and fungal microflora during combine and manual threshing of seeds. When manually threshed, seeds are dominated by bacterial microflora with a predominance of the genus Bacillus. The fungal microflora is mainly dominated by representatives of the genus Penicillium. Fungal microflora prevails on seeds at combine threshing (310 thousand CFU/g), the main share of which is represented by micromycetes of the genus Aspergillus (120 thousand КОЕ/g) and various yeast species (190 thousand CFU/g), which contributes to the accumulation of mycotoxins and accompanied by loss of seed germination (up to 86%). Application of crop desiccation at combine threshing provides the formation of a microbial community with predominance of micromycetes of Penicillium genus (66%), which allows to keep seed germination at the level of 95% throughout the whole storage period. A significant reserve for increasing the sowing properties of seeds is the desiccation of crops with post-harvest treatment, which reduces damage and ensures the formation of epiphytic microflora, which prevents the development of molds, which negatively affect the sowing properties of seeds.
About the Authors
N. S. ShimanskayaRussian Federation
Natalia S. Shimanskaya, CSc(Ag), Leading Research Associate
17/56 komsomolskiy Ave., Tver, 170041
S. V. Ivanova
Russian Federation
Svetlana V. Ivanova, Junior Research Associate
21 Nakhimova St., Smolensk, 214025
V. A. Serkov
Russian Federation
Valerian A. Serkov, DSc (Ag), Chief Research Associate
1B Michurina St., Lunino w.s., Penza region, 442731
I. V. Uschapovsky
Russian Federation
Igor V. Uschapovsky, CSc(Bio), Deputy Director for Research
17/56 komsomolskiy Ave., Tver, 170041
References
1. Myakin V.N., Uryupin S.G. Seed damaging as result of post-harvest treatment and ways of its reduction. Tekhnicheskie nauki. 2006:73-75. (In Russ.)
2. Elias S.G., Wu Y. – Сh., Stimpson D.C. Seed Quality and Dormancy of Hemp (Cannabis sativaL.).JournalofAgriculturalHempResearch.2020;2(1):2.https://doi.org/10.61611/2688-5182.1017
3. Orobinsky V.I., Gievsky A.M., Chernyshov A.V., Baskakov I.V. Research of qualitative indicators of winter wheat seeds during combine harvesting and postharvest processing. Transactions of Taurida Agricultural Science. 2020;21(184):84-97. (In Russ.)
4. Gimadiev A.M. Seed damaging. Agroinform. 2010;6:18-19. (In Russ.)
5. Pekhalskiy I.A., kryazhkov V.M.,Artyushin A.A., Sorochinskiy V.F.Traumatizing of inside structures of weevils as a factor of reduction in seeds’ productivity of cereal crops. Polythematic Online Scientific Journal of Kuban State Agrarian University. 2016:03(117):783-792. (In Russ.)
6. FGBU “Rosselkhotsentr” for kursk region. (In Russ.) [Electronic source]. URL: https://rosselhoscenter-kursk.ru (accessed: January 15, 2024)
7. Stepanova I.V. Investigation of factors affecting the safety of cannabis seeds: CSc (Ag) thesis. Moscow, USSR, 1974:22. (In Russ.)
8. Burkin A.A.,Ustyuzhanina M.I.,Zotova E.V.,kononenko G.P.Reasonsofcontamination of production lots of sunflower (Helianthus annuus L.) Seeds by mycotoxins. Agricultural Biology. 2020;5(53):969-976. (In Russ.) https://doi.org/10.15389/agrobiology.2018.5.969rus
9. Pesticides. (In Russ.) [Electronic source]. URL: https://www.pesticidy.ru/pesticide/regulyat-super
10. GOST R52325-2005. Seeds of agricultural plants. Sorting and sowing qualities. General technical conditions. Introduced 01.01.2006. Moscow, Russia: Standardinform, 2005:20. (In Russ.)
11. Gritsenko V.V., kaloshina Z.M. Seed science of field crops. 3d ed., updat. and rev. Moscow, USSR: kolos, 1984:272. (In Russ.)
12. Segi I. Soil microbiology techniques. Moscow, USSR: kolos, 1983:253. (In Russ.)
13. Tepper E.Z., Shilnikova V.k., Pereverzeva G.I. Microbiology practical course. Moscow, Russia: kolos, 2005:256. (In Russ.)
14. Stonov L.D., Stonov L.D., Zubkova N.F. Modern problems of defoliation and desiccation. Zashchita rasteniy. 1978;8:24-28. (In Russ.)
15. Efimochkina N.R., Sedova I.B., Sheveleva S.A., Tutelyan V.A. Toxigenic properties of mycotoxin-producing fungi. Tomsk State University Journal of Biology. 2019;45:6-33. (In Russ.) https://doi.org/10.17223/19988591/45/1
16. Nazarko M.D., Ocheredko N.S. The influence of mycotoxins on the quality of sunflower seeds. Izvestiya Vuzov. Food Technology. 2006;2(3):109-110. (In Russ.)
17. Punja Z.k., Ni L., Lung S., Buirs L. Total Yeast and Mold Levels in High THC-containing Cannabis (Cannabis sativa L.) Inflorescences are Influenced by Genotype, Environment, and Preand Post-harvest Handling Practices. Front. Microbiol. 2023;14:1192035. https://doi.org/10.3389/fmicb.2023.1192035
18. Comeau D., Balthazar C., Novinscak A., Bouhamdani N. et al. Interactions Between Bacillus Spp., Pseudomonas Spp. and Cannabis sativa Promote Plant Growth. Front Microbiol. 2021;12:715758. https://10.3389/fmicb.2021.715758
19. Dumigan C.R., Deyholos M.k. Cannabis Seedlings Inherit Seed-Borne Bioactive andAnti-FungalEndophyticBacilli.Plants.2022;11(16):2127.https://doi.org/10.3390/plants11162127
20. Bakulova I.V., Pluzhnikova I.I., kriushin N.V. Sowing qualities of seeds and productivity of non-narcotic variety of hemp depending on pre-planting cultivation. Niva Povolzhya. 2020;2(55):71-76. (In Russ.) https://doi.org/10.36461/NP.2020.2.55.012
Review
For citations:
Shimanskaya N.S., Ivanova S.V., Serkov V.A., Uschapovsky I.V. Problem of reducing the quality of hemp seeds and ways to solve it. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2024;(5):58-67. (In Russ.) https://doi.org/10.26897/0021-342X-2024-5-58-67