Preview

IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY

Advanced search

Study of the adaptability of forest berry plants ex vitro to the soil and climatic conditions of the Vologda region

https://doi.org/10.26897/0021-342X-2024-4-36-52

Abstract

The article presents the results of studies on phenological and morphological characteristics of forest berry plants obtained by microclonal propagation and adapted ex vitro, after transplanting to open ground conditions in the Vologda district of the Vologda region. Nowadays, in the conditions of import substitution and demand for fruit and berry products, the industrial cultivation of berry planting material is necessary. The objects of research are lingonberry (Vaccinium vitis-idaea L.) cultivars ‘Kostromichka’ and ‘Kostromskaya Rozovaya’, lowbush blueberry (Vaccinium angustifolium Ait.) cultivars ‘Northblue’ and ‘Northcountry’, European cranberry (Vaccinium oxycoccos L.) cultivars ‘Dar Kostromy’ and ‘Severyanka’, arctic bramble (Rubus arcticus L.) cultivars ‘Astra’ and ‘Galina’. The winter hardiness of 2-year-old seedlings of the studied berry cultivars obtained by the in vitro method after overwintering was 89–100% in the first decade of May. The average height of 2-year-old R. arcticus plants is 9.3 to 10.2 cm, V. angustifolium – 17.4 to 18.1 cm, V. oxococcos – 6.4 to 7.2 cm, V. vitis-idaea – 6.2 to 7.0 cm. The yield of air-dry phytomass of leaves of 2-year-old R. arcticus plants averaged 112.9 to 151.8 g/m2, V. vitis-idaea – 2.1 to 2.2 g/m2; the air-dry mass of plant leaves was 18 to 21% of the wet mass. Leaf damage by the cruciferous flea beetle (Phyllotreta Stephens) was observed on 2-year-old R. arcticus plants. The results obtained indicate a rather high adaptability of the studied berry plants to the soil and climatic conditions of the Vologda region, Russia.

About the Authors

E. I. Kulikova
Vologda State Dairy Farming Academy named after N.V. Vereshchagin
Russian Federation

Elena I. Kulikova, CSc (Ag), Associate Professor, head of the Department of Plant Growing, Agriculture and Agrochemistry

2 Schmidta St., Molochnoe, Vologda, Vologda region, 160555, phone: (172) 52–57–30
 



L. V. Zarubina
Vologda State Dairy Farming Academy named after N.V. Vereshchagin
Russian Federation

Lilia V. Zarubina, DSc (Ag), Associate Professor, Professor at the Department of Forestry

 2 Schmidta St., Molochnoe, Vologda, Vologda region, 160555, phone: (172) 52–57–30 



V. V. Surov
Vologda State Dairy Farming Academy named after N.V. Vereshchagin
Russian Federation

Vladimir V. Surov, PhD (Ag), Associate Professor, Associate Professor at the Department of Plant Growing, Agriculture and Agrochemistry

2 Schmidta St., Molochnoe, Vologda, Vologda region, 160555, phone: (172) 52–57–30



D. V. Donya
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation

Denis V. Donya, CSc (Eng), Associate Professor at the Department of Processes and Equipment of Processing Industries

49 Timiryazevskaya St., Moscow, 127550, phone: (499) 977–92–73 



Yu. V. Ustinova
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation

Yulia V. Ustinova, CSc (Eng), Associate Professor at the Department of Technology of Storage and Processing of livestock Products

49 Timiryazevskaya St., Moscow, 127550, phone: (499) 976–46–12 



N. S. Umnov
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation

Nikolay S. Umnov, postgraduate student, Assistant at the Department of landscape Architecture

49 Timiryazevskaya St., Moscow, 127550, phone: (499) 976–12–43 



References

1. Agroclimatic resources of the Vologda region. Leningrad, USSR: Gidrometeoizdat, 1972:185. (In Russ.)

2. Makarov S.S., Kuznetsova I.B., Rodin S.A. et al. Adaptation of European cranberry to non-sterile conditions with the addition of organic products and hormones. Sibirskiy lesnoy zhurnal. 2022;1:52–60. (In Russ.) https://doi.org/10.15372/SJFS20220105

3. Makarov S.S., Samoilenko Z.A., Makarova T.A. et al. Adaptation of American cranberry (Vaccinium macrocarpon Ait.) to ex vitro conditions using the hydroponic method. Bulletin of KSAU. 2023;11:104–112. (In Russ.) https://doi.org/10.36718/1819-4036-2023-11-104-112

4. Makarov S.S., Chudetsky A.I., Tyak G.V. et al. Adaptation of forest berry plants to non-sterile conditions in vivo using modern biological products. Forestry Information. (In Russ.) 2021;3:84–91. https://doi.org/10.24419/LHI.2304-3083.2021.3.07

5. Makarov S.S., Upadyshev M.T., Rodin S.A. et al.Adaptation of regenerated plants of Rubus arcticus L. to ex vitro conditions using hydroponics. Sibirskiy lesnoy zhurnal. 2023;4:75–82. (In Russ.) https://doi.org/10.15372/SJFS20230408

6. Fedorenko V.F., Mishurov N.P., Kondratieva O.V. et al. Analysis of the state and prospects for the development of nursery farming and horticulture: a scientific analytical review. Moscow, Russia: Rosinformagrotekh, 2019:88. (In Russ.)

7. Makarov S.S., Antonov A.M., Kulikova E.I. et al. Biotechnology in horticulture. Growing fruit and rare berry plants in in vitro culture: Laboratory workshop. St. Petersburg, Russia: Lan’, 2023:128. (In Russ.)

8. Butenko R.G. Biology of cells of higher plants in vitro and biotechnologies based on them. Moscow, Russia: FBK-Press, 1999:160. (In Russ.)

9. Makarov S.S., Rodin S.A., Kuznetsova I.B. et al. Effect of light on rhizogenesis of forest berry plants during clonal micropropagation. Food Processing: Techniques and Technology. 2021;51(3):520–528. (In Russ.) https://doi.org/10.21603/2074-9414-2021-3-520-528

10. Makarov S.S., Kulikova E.I., Kuznetsova I.B. et al. Influence of nutrition medium composition on root formation of bog blueberry (Vaccinium uliginosum L.) of Northern Russian origin in vitro culture. Bulletin of KSAU. 2023;12:121–127. (In Russ.) https://doi.org/10.36718/1819-4036-2023-12-121-127

11. Gegechkori B.S., Doroshenko T.N., Shcherbakov N.A. Innovative technologies for the production of planting material for fruit and berry crops: textbook. St. Petersburg, Russia: Lan’, 2022:208. (In Russ.)

12. Dospekhov B.A. Methods of field experience (with the basics of statistical processing of research results): textbook. Moscow, Russia: Al’yans. 2011:350. (In Russ.)

13. Chudetsky A.I., Zaushintsena A.V., Rodin S.A. et al. The use of modern growth-promoting eco-preparations for microclonal propagation of lingonberry (Vaccinium vitis-idaea L.). Forestry Information. 2022;2:56–66. (In Russ.) https://doi.org/10.24419/LHI.2304-3083.2022.2.05

14. Kobysheva N.V., Akent’eva E.M., Bogdanova E.G. et al. Climate of Russia: monograph. St. Petersburg, Russia: Gidrometeoizdat, 2001:654. (In Russ.)

15. Makarov S.S., Upadyshev M.T., Sungurova N.R. et al. Clonal micropropagation of wild berry plants of the genus Rubus. Food Processing: Techniques and Technology. 2024;54(1):60–70. (In Russ.) https://doi.org/10.21603/2074-9414-2024-1-2488

16. Korenev I.A., Tyak G.V., Makarov S.S. Creation of new varieties of forest berry plants and prospects of their intensive reproduction (in vitro). Forestry Information. 2019;3:180–189. (In Russ.) https://doi.org/10.24419/LHI.2304-3083.2019.3.15

17. Kuznetsova I.B., Makarov S.S. Influence of auxin IAA concentration and the Ecogel preparation on rhizogenesis of European cranberry (Oxycoccus palustris Pers.) in vitro. Vestnik Buryatskoy gosudarstvennoy selskhokhozyasvennoy akademii imeni V.R. Filippova. 2022;1(66):99–104. (In Russ.) https://doi.org/10.34655/bgsha.2022.66.1.013

18. Kuznetsova I.B., Chudetsky A.I., Tyak G.V. Influence of light on the shoot formation and rhizogenesis of cowberry in clonal micropropagation. Vestnik Buryatskoy gosudarstvennoy selskhokhozyasvennoy akademii imeni V.R. Filippova. 2021;3(64):102–108. (In Russ.) https://doi.org/10.34655/bgsha.2021.64.3.013

19. Kuznetsova I.B.,Makarov S.S.Influenceofnutrientmediumandgrowth-regulating substances on root formation of European cranberry (Oxycoccus palustris Pers.) in vitro. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta. 2021;6(92):99–103. (In Russ.) https://doi.org/10.37670/2073-0853-2021-92-6-99-103

20. Kuznetsova I.B., Makarov S.S., Abdurasuli B. The influence of growth regulating substances on the organogenesis of regenerated plants on the stage actually micropropagation cloning crops. Pomiculture and Small Fruits Culture in Russia. 2016;47:198–202 (In Russ.)

21. Makarov S.S., Kalashnikova E.A., Kirakosyan R.N. Vegetative reproduction of blue honeysuckle (Loniceria ceruleae L.) in vivo and in vitro. Izvestiya Timiryazevskoy selskokhozyaystvennoy akademii. 2018;1:82–91. (In Russ.) https://doi.org/10.26897/0021-342X-2018-1-82-91

22. Makarov S.S., Kuznetsova I.B. Influence of growth regulators on organogenesis of honeyberry when clonic micropropagation. Bulletin of NSAU (Novosibirsk State Agrarian University). 2018;4:36–42. (In Russ.) https://doi.org/10.31677/2072-6724-2018-49-4-36-42

23. Makarov S.S., Kuznetsova I.B., Smirnov V.S. Effect of growth regulators on the organogenesis of plants when the clonal micropropagation of arctic bramble (Rubus arcticus L.). Forestry Information. 2017;2:103–108. (In Russ.) https://doi.org/10.24419/LHI.2304-3083.2017.2.10

24. Makarov S.S., Kuznetsova I.B., Klevtsov D.N. Influence of growth-regulating substances on organogenesis of arctic bramble (Rubus arcticus L.) plants during clonal micropropagation. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta. 2021;3(89):88–92. (In Russ.) https://doi.org/10.37670/2073-0853-2021-89-3-88-92

25. Makarov S.S., Kalashnikova E.A. Influence of nutrient medium composition on clonal micropropagation of honeysuckle edible. Pomiculture and small fruits culture in Russia. 2017;49:217–222. (In Russ.)

26. Makarov S.S., Kuznetsova I.B. Clonal micropropagation of a half-high blueberry at the stages of “introduction to culture” and “micropropagation proper”. Vestnik Buryatskoy gosudarstvennoy selskhokhozyasvennoy akademii imeni V.R. Filippova. 2019;3:28–33. (In Russ.) https://doi.org/10.34655/bgsha.2019.56.3.004

27. Makarov S.S., Tyak G.V. Arctic bramble (Rubus arcticus L.): development of a method for testing for distinctiveness, homogeneity and stability. Vestnik Kurskoy gosudarstvennoy selskhokhozyasvennoy akademii. 2023;7:79–85 (In Russ.)

28. Makarov S.S., Kuznetsova I.B. In vitro root formation and ex vitro adaptation of arctic bramble during clonal micropropagation. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta. 2018; 6:52–55 (In Russ.)

29. Makarov S.S., Rodin S.A., Chudetsky A.I. Guidelines for growing planting material of forest berry crops in vitro and in vivo. Pushkino, Russia: All-Russian Research Institute of Silviculture and Mechanization of Forestry, 2019:24. (In Russ.)

30. Makarov S.S. Scientific and methodological substantiation of the technology of propagation and plantation cultivation of forest berry plants. DSc (Ag) thesis. Pushkino, Russia, 2022:467. (In Russ.)

31. Makarov S.S., kuznetsova I.B., Smirnov V.S. Organogenesis of half-highbush blueberry during clonal micropropagation depending on lighting conditions. Izvestiya orenburgskogo gosudarstvennogo agrarnogo universiteta. 2021;4:76–79. (In Russ.) https://doi.org/10.37670/2073-0853-2021-90-4-76-79

32. Makarov S.S., kalashnikova E.A., Rumyantseva E.P. Productivity of edible honeysuckle depending on the technology of propagation. Vesting of Volga State University of Technology. Series: Forest. Ecology. Nature Management. 2018;3:76–83. (In Russ.) https://doi.org/10.15350/2306-2827.2018.3.76

33. Makarov S.S. Development of technology for clonal micropropagation of forest berry plants and its introduction into cultivation on depleted peatlands. CSc (Ag) thesis. Pushkino, Russia, 2019: 132. (In Russ.)

34. Makarov S.S., kuznetsova I.B., Smirnov V.S. Improving technology of clonal micropropagation of arctic bramble (Rubus arcticus l.). Forestry Information. 2018;4:91–97 (In Russ.)

35. Makeeva G.Yu., Tyak G.V., Makeev V.A., Makarov S.S. Creation of the first Russian cultivars of blueberry (Vaccinium angustifolium Ait.). Contemporary Horticulture. 2023;1:1–14. (In Russ.) https://doi.org/10.52415/23126701_2023_0101

36. Vladimirov D.R.,Gladilin A.A.,Gnedenko A.E.etal.Methodologyforconducting phenological observations. Moscow, Russia: Alpina Pro, 2023:208. (In Russ.)

37. Methodology for determining reserves of medicinal plants. Moscow, USSR: CBNTI leskhoza, 1986:52. (In Russ.)

38. Makarov S.S., Chudetsky A.I., Rodin S.A., kulikova E.I. Methodological recommendations for growing planting material of forest berry plants in in vitro culture. Pushkino, Russia: All-Russian Research Institute of Silviculture and Mechanization of Forestry, 2023:32. (In Russ.)

39. Chudetsky A.I., Rodin S.A., Zarubina l.V. et al. Clonal micropropagation and peculiarities of adaptation to ex vitro conditions of forest berry plants of the genus Vaccinium. Food Processing: Techniques and Technology. 2022;52(3):570–581. (In Russ.) https://doi.org/10.21603/2074-9414-2022-3-2386

40. Review of agrometeorological conditions for the growth and development of agricultural crops in the Vologda region. Vologda, Russia: Vologodskiy tsentr po gidrometeorologii i monitoringu okruzhayushchey sredy, 2021:15. (In Russ.)

41. Chudetsky A.I., Makarov S.S., kuznetsova I.B. et al. Organogenesis of hybrid varieties of cowberry of the Russian selection in vitro depending on the composition of the nutrient medium and growth-regulating substances. Vestnik Buryatskoy gosudarstvennoy selskhokhozyasvennoy akademii imeni V.R. Filippova. 2023;1:141–149. (In Russ.) https://doi.org/10.34655/bgsha.2023.70.1.017

42. Makarov S.S., kuznetsova I.B., Upadyshev M.T. et al. Clonal micropropagation of cranberry (Oxycoccus palustris Pers.). Food Processing: Techniques and Technology. 2021;51(1):67–76. (In Russ.) https://doi.org/10.21603/2074-9414-2021-1-67-76

43. Kulikova E.I., Makarov S.S., kuznetsova I.B., Chudetsky A.I. Russian and foreign cultivars of honeysuckle (lonicera edulis Turcz.) cultivation studies in vitro. Food Processing: Techniques and Technology. 2021;51(4):712–722. (In Russ.) https://doi.org/10.21603/2074-9414-2021-4-712-722

44. Makarov S.S., Upadyshev M.T., khamitov R.S. et al. Prospects for industrial cultivation and biotechnological methods of propagation of forest berry plants. Moscow, Russia: kolos-S, 2023:152. (In Russ.)

45. Chudetsky A.I., Babich N.A., Melekhov V.I. et al. Prospects for industrial cultivation and biotechnological methods of propagation of forest berry plants of the genus Vaccinium (lingonberry, Kamchatka bilberry). Moscow, Russia: kolos-S, 2023:184. (In Russ.)

46. Makarov S.S., kuznetsova I.B., Zaushintsena A.V. et al. Improving the efficiency of multipurpose forest management on depleted peatlands. Russian Forestry Journal. 2022;3:91–102. (In Russ.) https://doi.org/10.37482/0536-1036-2022-3-91-102

47. Makarov S.S., Tyak G.V., kuznetsova I.B. et al. Obtaining planting material of Rubus arcticus l. by clonal micropropagation. Russian Forestry Journal. 2021;6(384):89–99. (In Russ.) https://doi.org/10.37482/0536-1036-2021-6-89-99

48. Makarov S.S., Upadyshev M.T., kuznetsova I.B. et al. The use of lighting ofvariousspectralrangesforclonalmicropropagationofforestberryplants.RussianForestry Journal. 2022;6(390):82–93. (In Russ.) https://doi.org/10.37482/0536-1036-2022-6-82-93

49. Makarov S.S., Bagaev E.S., Tsaregradskaya S.Yu., kuznetsova I.B. Problems of use and reproduction of phytogenic food and medicinal forest resources on the forest fund lands of the kostroma region. Russian Forestry Journal. 2019;6:118–131. (In Russ.) https://doi.org/10.37482/0536-1036-2019-6-118

50. Sedov E.N., Ogoltsova T.P. (eds.). Program and methodology for studying varieties of fruit, berry and nut crops. Orel, Russia: All-Russian Research Institute of Fruit Crop Breeding, 1999:606. (In Russ.)

51. Tyak G.V., Makarov S.S., kalashnikova E.A., Tyak A.V. Reproduction and cultivation of the arctic bramble (Rubus arcticus l.). Pomiculture and small fruits culture in Russia. 2018;52:95–99. (In Russ.)

52. Tyak G.V., kurlovich l.E., Makarov S.S. et al. Reproduction of promising hybrid forms of lingonberry (Vaccinium vitis-idaea l.). Vestnik Buryatskoy gosudarstvennoy selskhokhozyasvennoy akademii imeni V.R. Filippova. 2022;1(66):113–118. (In Russ.) https://doi.org/10.34655/bgsha.2022.66.1.015

53. Tyak G.V., l.E. kurlovich, Makarov S.S. et al. Recommendations for selecting methods for obtaining planting material of forest berry plants for cultivation on non-forest lands. Pushkino, Russia: All-Russian Research Institute of Silviculture and Mechanization of Forestry, 2023:24. (In Russ.)

54. Makarov S.S., Babich N.A., kulikova E.I. et al. Rhizogenesis of narrow-leaved blueberry (Vaccinium angustifolium Ait.) in vitro depending on the concentration of auxins. Forestry Information. 2022;1:74–84. (In Russ.) https://doi.org/10.24419/lhI.2304-3083.2022.1.05

55. Migulin A.A. et al. (comps.); Migulin A.A., Osmolovsky G.E. (eds.). Agricultural entomology. Moscow, USSR: kolos, 1976:447. (In Russ.)

56. Makarov S.S., Vinogradova V.S., Tyak G.V., Babich N.A. Theory and practice of propagation and plantation cultivation of forest berry plants as a Rubus arcticus L., oxycoccus palustris Pers. and Vaccinium angustifolium Ait. karavaevo, Russia: kostroma State Agricultural Academy, 2021:394. (In Russ.)

57. Makarov S.S., Feklistov P.A., kuznetsova I.B. et al. Technologies for propagation and cultivation of blueberry species and varieties to create a bioresource collection. Achievements of Science and Technology in Agro-Industrial Complex. 2023;37(12):11–16. (In Russ.) https://doi.org/10.53859/02352451_2023_37_12_11

58. Tyak G.V., Makarov S.S. Introduction of arctic bramble (Rubus arcticus l.). Plodovodstvo, semenovodstvo, introduktsiya drevesnykh rasteniy. 2021;24:163–166 (In Russ.)

59. Tyak G.V., kurlovich l.E., Makarov S.S. Reproduction of hybrid forms of lowbush blueberry with lignified cuttings. Forestry Information. 2022;3:95–104. (In Russ.) https://doi.org/10.24419/lhI.2304-3083.2022.3.08

60. Chudetsky A.I., Makarov S.S., Rodin S.A. et al. Rooting in vitro and adaptation to non-sterile conditions of Russian selection cultivars of lingonberry. Forestry Information. 2023;2:102114. (In Russ.) https://doi.org/10.24419/lhI.2304-3083.2023.2.08

61. Chudetsky A.I., Makarov S.S., Rodin S.A. Guidelines for growing lingonberry and Kamchatka bilberry planting material in vitro and ex vitro. Pushkino, Russia: All-Russian Research Institute of Silviculture and Mechanization of Forestry, 2022:20. (In Russ.)

62. Chudetsky A.I. Development of technology for clonal micropropagation of forest berry plants of the genus Vaccinium for plantation cultivation on non-forest lands of the forest fund. CSc (Ag) thesis. Pushkino, Russia, 2022:208. (In Russ.)

63. Makarov S.S., kuznetsova I.B., Chudetsky A.I., Rodin S.A. Obtaining high-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands. Russian Forestry Journal. 2021;2:21–29. https://doi.org/10.17238/0536-1036-2021-2-21-29


Review

For citations:


Kulikova E.I., Zarubina L.V., Surov V.V., Donya D.V., Ustinova Yu.V., Umnov N.S. Study of the adaptability of forest berry plants ex vitro to the soil and climatic conditions of the Vologda region. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2024;(4):36-52. (In Russ.) https://doi.org/10.26897/0021-342X-2024-4-36-52

Views: 156


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0021-342X (Print)