Microclonal reproduction of Digitalis purpurea L. And adaptation of regenerants in hydroponics

The paper presents the results of the development of the microclonal propagation technology of Digitalis purpurea L. with plant adaptation in hydroponic Ebb and Flow Systems. It has been found that MS and WPM growth media with a full and reduced content of mineral salts supplemented with 6-BAP cytokinines, 2-iP and auxins of indoleacetic acid, isobutyric acid in various concentrations can be highly efficient for in vitro cultivation of the plants. Intensive shoot proliferation and rhizogenesis of plants in vitro culture is observed on MS and WPM growth media with a twofold reduced content of mineral salts, with 6-BAP phytohormones 0.5 mg/L and 2-iP 0.3 mg/L. A high frequency of callus formation was observed on MS growth medium with full mineral salt composition containing 6-BAP 0.5 mg/L. The use of hydroponic systems at the stage of adaptation to ex vitro conditions ensures high survival of plants. During the work, anatomical and morphological features of regenerating plants under in vitro and ex vitro conditions were studied.

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