Molecular genetic identification and certification of sugar beet hybrids using microsatellite markers

The development of molecular genetic identification and certification of sugar beet hybrids using DNA markers is a current research direction in plant breeding. The subject of the study were plants of promising domestic sugar beet hybrids. The article presents the results of the development of the methodology of molecular genetic identification and certification of sugar beet hybrids using microsatellite markers. Seedlings of eight prospective domestic sugar beet hybrids were used as test material. Target fragments were amplified using locus-specific (microsatellite) primers (Unigene16898, Unigene17623B, Unigene26753, Unigene17923, Unigene27833, FDSB1001, FDSB1033, FDSB502–2, FDSB502–3, SB09, SB04, and SB15) labeled with fluorescent dyes (FAM, R6G, TAMRA and Rox). The obtained amplicons were identified by the method of high-resolution capillary electrophoresis using the genetic analyzer Nanofor 05 (Synthol, Russia). The results of selection of 12 microsatellite loci suitable for genetic certification of sugar beet hybrids are presented. All primers selected for microsatellite loci of sugar beet genome are characterized by a high level of polymorphism. From the results of fragment analysis, the size of the obtained PCR amplicons in plants of the studied hybrids was determined. on the basis of the obtained molecular data, genetic formulas and passports for identification and certification of plant genotypes were prepared. Based on the calculated genetic distances, a dendrogram of genetic relationships of the studied sugar beet hybrids was constructed. The development of methods for molecular genetic identification and certification of sugar beet hybrids using DNA markers is a current research direction in plant breeding. It is of great practical importance in sugar beet breeding, seed production and state variety testing, both in the development of new hybrids and in their registration.

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