Factors affecting gynogenesis induction in cucumber (Cucumis sativus L.) through ovary culture

Improving the efficiency of doubled haploid technology is an urgent task to expand the possibilities of fundamental research and to increase the selection rate of commercial F1 hybrids. This study investigated the effect of the components of the induction nutrient medium: casein hydrolyzate ( 250 mg/l and 500 mg/l ) , glutathione  ( 10 mg/l) , a combination of growth regulators TDZ  ( 0.04 mg/l)  and 2,4-D ( 0.15 mg/l)  on the gynogenic response of cucumber in unfertilized ovules culture. The results showed that the addition of 250 mg/l of casein hydrolysate to the induction medium resulted in a more than twofold increase in the frequency of gynogenic ovules in two samples. The addition of 10 mg/l of glutathione to the induction medium helps to increase the frequency of gynogenic ovules by 1.5 to 2 times in 3 out of 6 samples. The results showed that cultivation of cucumber ovary fragments on the induction medium supplemented with 0.5 mg/l of putrescine leads to decrease in frequency of gynogenic ovules. The obtained data on the influence of the components of the induction medium in the culture of ovules can be used to optimize the technology for the production of doubled haploids in cucumber.

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