Experience of growing Daphnia magna on the basis of chlorella in small containers with standing water

Industrial fish farming is the most dynamically developing area of domestic aquaculture. Therefore, the problem of obtaining live feeds for growing juvenile fish under artificial conditions is of utmost importance, since such feeds are characterized by high nutritional value, high content of protein, fat, essential amino acids, vitamins, enzymes and other important components. One of the most commonly used zooplanktonic species for feeding juvenile fish is Daphnia magna, which is characterized by high fertility, rapid growth, adaptability to a wide range and changes in abiotic environmental factors. However, in order to obtain consistently high results from the cultivation of these organisms, it is necessary to create a specific set of conditions in each individual case. The results of growing Daphnia magna for food purposes in small containers with standing water are presented. At the same time, an experiment was carried out to select the best possible feeding option. Glass containers with three liters of water were used; the density of the Daphnia planting was 2000 pcs/l. The water temperature was maintained at +19 to +21°C. Artificial lighting was provided for 14 hours per day. The water was changed once every two days for 70% of the initial volume. The duration of the test was 14 days. In all experimental variants, the ammonia content of nitrites and nitrates did not exceed the MPC. The best growth rates were obtained when the Daphnia were fed with chlorella culture compared to feeding with yeast or a mixture of yeast and chlorella. The use of a chlorella-only diet reduced water turbidity and increased Daphnia survival rate of by 17–25%, and feed yield by 23.6–39.1% compared to other experimental options.

1. Bogdanov N.I., Asanov A.Yu. Pond fish farming, – 3rd ed., supplement – Penza, 2011. – 89 s. (In Rus.)

2. Ivleva I.V. Biological bases and methods of mass cultivation of forage invertebrates. Moscow: Nauka, 1969. – 171 s. (In Rus.)

3. Kalaida M.L. Biological foundations of fish farming: studies. stipend. Kazan: Kazan State Energy University, 2017. 148 s. (In Rus.)

4. Kamilov B.G., Yuldashov M.A. Aquaculture: textbook. Tashkent: LESSON PRESS, 2020. 412 s. (In Rus.)

5. Kozhokaru, T.T. On the issue of directional formation of the natural forage base of outgrowth ponds / T.T. Kozhokaru, V.N. Ulyanov, P. Dermenzhi // Fish farming and fisheries. – 2012. – No. 3. – S. 59-63. (In Rus.)

6. Mityaev A.V., Presnyakova E.V., Semenova E. F., Gurina M.A. Comparative analysis of producer and innovative product strains as the main elements of chlorella resinoid biotechnology // IVOZ PR Natural Sciences. 2014. No.4 (8). – S. 19-28. (In Rus.)

7. Rutkin, N.M. Lagutkina L.Yu., Lagutkin O.Yu. Urbanized agricultural production (city farming) as a promising direction for the development of global agricultural production and a way to improve the food security of cities // Bulletin of the Astrakhan State Technical University. Series: Fisheries. – 2017. – No. 4 – S. 95 – 103. (In Rus.)

8. Frolova M.V., Moskovets M.V., Ptitsyna L.A., Toropov A.Yu. Features of the influence of Chlorella vulgaris strain IGF No.C-111 on water quality in pond fish farming // Irrigated agriculture. – 2019. – No. 3. – S. 46-49. (In Rus.)

9. Sharopova Sh.R. Determination and breeding of dominant phyto- and zooplankton species, recommendations on biotechnology // 2022. S. 317-320. (In Rus.)

10. Sheikhgasanov K.G. Lagutkina L.Yu., Ponomarev S.V. The use of organic environmentally friendly biotechnology for growing fish and agricultural crops // Bulletin of the Astrakhan State Technical University. Series: Fisheries. – 2014. – No. 3. – S. 97-103. (In Rus.)

11. Abo-Taleb, H.A.; Ashour, M.; Elokaby, M.A.; Mabrouk, M.M.; El-feky, M.M.M.; Abdelzaher, O.F.; Gaber, A.; Alsanie, W.F.; Mansour, A.T. Effect of a New Feed Daphnia magna (Straus, 1820), as a Fish Meal Substitute on Growth, Feed Utilization, Histological Status, and Economic Revenue of Grey Mullet, Mugil cephalus (Linnaeus 1758). Sustainability 2021, 13, 7093. https://doi.org/10.3390/su13137093

12. Begum M., Noor P., Ahmed K.N., Mohanta L.C., Sultana N., Hasan M.R., Uddin M.N. Assessment of four different media for the mass culture of Ceriodaphnia reticulata (Jurine) as a live fish feed. Journal of the Asiatic Society of Bangladesh, Science 2013. 39(2):129-138.

13. Din M. U., W. and Altaff K. Culture of zooplankton for rearing fish larvae // Poll Res. 2010. 29 (2): 91-93.

14. Kar S., Das P., Das U., Bimola M., Kar D., Aditya G. Culture of the zooplankton as fish food: observations on three freshwater species from Assam, India. AACL Bioflux. 2017. 10(5):1210-1220.

15. Rahman H., Azani N., Suhaimi H., Yatim S. R., Yuslan A. and Rasdi N.W.A Review on Different Zooplankton Culturing Techniques and Common Problems Associated with Declining Density // IOP Conf. Ser.: Earth Environ. Sci., 2023. 1147 012012. DOI 10.1088/1755-1315/1147/1/012012

16. Rottmann R. W., Graves J. S., Yanong R. P. E. Culture Techniques of Moina: The Ideal Daphnia for Feeding to Freshwater Fish Fry // Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, 1992. 1-6.