Genetic diversity of varieties and lines of naked oats of the selection of FRC “Nemchinovka”
https://doi.org/10.26897/0021-342X-2023-5-62-74
Abstract
To assess the control of biotype constancy of naked oat varieties during cultivation, a highly effective method of electrophoresis of spare seed proteins – prolamins– is increasingly used in primary seed production. The aim of the research was to certify naked oatvarieties of the FRC “Nemchinovka” selection and to analyze their genetic diversity using alleles of avenin-coding loci.Chemical-technological, computational-constructive and statistical research methods were used in the work. The method of electrophoresis of spare alcohol–soluble proteins of oats – avenins – was used. Electrophoresis was carried out in vertical plates of 13.2% polyacrylamide gel at a constant voltage of 500 V using VE-20 electrophoretic chambers (Helicon, Russia). As a result of the conducted studies, it was found that all the analyzed samples were heterogeneous in the composition of avenin components, with the number of biotypes ranging from 2 to 14 pcs. The frequency of occurrence of biotypes in the samples varied from 1.0 to 97.0%. The blocks of avenin components missing in the catalog of genetic nomenclature were found: four blocksby Avn A locus, two blocksby Avn B locus, and one blockby Avn C locus. Their probable component composition was determined. A high value of the average gene diversity was found for all three loci. This indicates the effective use of genetically diverse source material in the breeding process.
About the Authors
A. D. Kabashov
Federal Research Center “Nemchinovka”
Aleksandr D. Kabashov, CSc (Ag), Acting Head of the Laboratory of Breeding and Primary Seed Production of Oats
6, Agrokhimikov Str, Novoivanovskoe workers’settlement, Odintsovo, Moscow Region, 143026
A. V. Lyubimova
Federal Research Centre “The Tyumen Scientific Centre of the Siberian Branch of the Russian Academy of Sciences”
Anna V. Lyubimova, CSc (Bio), Head of the Laboratory
86, Malygina Str., Tyumen, 625026
N. M. Vlasenko
Federal Research Center “Nemchinovka”
Nikolay M. Vlasenko, CSc (Ag), Leading Research Associate, Laboratory of Breeding and Primary Seed Production of Oats
6, Agrokhimikov Str, Novoivanovskoe workers’settlement, Odintsovo, Moscow Region, 143026
A. S. Kolupaeva
Federal Research Center “Nemchinovka”
Russian Federation
Russian Federation
Anastasia S. Kolupaeva, Research Associate, Laboratory of Breeding and Primary Seed Production of Oats
6, Agrokhimikov Str, Novoivanovskoe workers’settlement, Odintsovo, Moscow Region, 143026
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Review
For citations:
Kabashov A.D., Lyubimova A.V., Vlasenko N.M., Kolupaeva A.S. Genetic diversity of varieties and lines of naked oats of the selection of FRC “Nemchinovka”. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2023;(5):62-74. (In Russ.) https://doi.org/10.26897/0021-342X-2023-5-62-74
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