Peculiarities of organogenesis of hard cultured low-growing cultivars of sour cherry (Prunus cerasus L.) in in vitro culture

The article presents the results of research on clonal micropropagation of sour cherry (Prunus cerasus L.) which is difficult to reproduce at the proliferation stage by traditional methods. A number of modern winter-hardy domestic cultivars of P. cerasus are promising for plantation cultivation of this crop in the Nonchernozem zone of the European part of Russia. The method of clonal micropropagation is advisable to obtain a large amount of healthy and genetically homogeneous rootstocks of fruit crops. Regenerated plants of P. cerasus of low-growing, mid-season and winter-hardy cultivars Assol’ and Shokoladnitsa were used as research objects. The growth and developmental features of P. cerasus regenerated plants were studied during in vitro cultivation on a QL nutrient medium supplemented with growth regulators of the cytokinin group (6-BAP, thidiazuron, zeatin) in different concentrations. The highest indicators for the length of microshoots (average 13.4 mm) and the number of leaves (average 6.0 pcs.) of P. cerasus plants in in vitro culture are recorded for the Shokoladnitsa cultivar. The maximum length of microshoots of P. cerasus Assol’ cultivar is observed on the 60th day of cultivation on a QL nutrient medium with the addition of zeatin at a concentration of 0.3 mg/l (10.5 mm) and thidiazuron at a concentration of 0.3 mg/l (9.8 mm), while the maximum number of leaves (7.8 pieces) is observed with addition of 0.3 mg/l zeatin. The maximum length of microshoots of P. cerasus of the Shokoladnitsa cultivar is observed when grown for 60 days on a QL nutrient medium with the addition of 6-BAP at concentrations of 0.5 mg/l (17.7 mm) and 1.0 mg/l (14.2 mm), while the maximum number of leaves (7.2 pieces) is observed with addition of 0.3 mg/l zeatin

1. Astakhov A.A. New varieties of sour cherry and sweet cherry for the South of the Non-Chernozem Region. Horticulture and Viticulture. 2007; 5: 21–23. (In Rus.)

2. Butenko R.G. Biology of cells of higher plants in vitro and biotechnology based on them. Moscow: FBK-Press, 1999: 160. (In Rus.)

3. Verzilin A.V. New variety-rootstock combinations of cherry. Contemporary Horticulture. 2016; 4: 19–24. URL: http://journal.vniispk.ru (In Rus.)

4. Vechernina N.A. Methods of biotechnology in breeding, reproduction and conservation of the plant gene pool. Barnaul: Izd-vo Altayskogo un-ta. 2004: 205. (In Rus.)

5. Vysotskiy V.A., Oleshko E.A. Improvement of the nutrient medium for clonal micropropagation of cherry. Agrotekhnika i sortoizuchenie plodovyh kul’tur. Moscow, 1985: 72–76. (In Rus.)

6. Demenko V.I., Trushechkin V.G. Cherry propagation by in vitro method. Agricultural Biology. 1983; 7: 51–53. (In Rus.)

7. Dzhigadlo M.I. Use of biotechnological and biophysical methods in selection and cultivar breeding of fruit and berry crops. CSc (Ag) thesis. Michurinsk, 2003: 26. (In Rus.)

8. Dospekhov B.A. Methods of field experience (with the basics of statistical processing of research results): Textbook. Moscow: Al’yans. 2011: 350. (In Rus.)

9. Kanshina M.V., Astakhov A.A., Misnikova N.V., Yagovenko G.L. Evaluation of the adaptability of sour cherry and sweet cherry cultivars in the South of the Non-Chernozem Region. Selektsiya i sortorazvedenie sadovykh kul’tur. 2021; 8; 1–2: 45–48. DOI: 10.24411/2500–0454–2021–10114 (In Rus.)

10. Kartashova O.N. Winter hardiness and productivity of new cherry cultivars in the conditions of the Non-Chernozem Region. CSc (Ag) thesis. Moscow, 2009: 26. (In Rus.)

11. Kovalenko N.N., Polivara N.V. Improvement of the method for obtaining free from saprophytic microflora embryos of the genus prunus L. for in vitro culture. Plodovodstvo i Vinogradarstvo Yuga Rossii. 2020; 63 (3): 107–120. DOI: 10.30679/2219–5335–2020–3–63–107–120 (In Rus.)

12. Krasinskaya T.A. Stabilization of explants of cherry cultivars in in vitro culture. Fruit-Growing. 2017; 29: 88–92. (In Rus.)

13. Kuznetsova L.A. Economic and biological features of new variety-rootstock combinations of cherry in the nursery. CSc (Ag) thesis. Michurinsk, 2011: 22. (In Rus.)

14. Metlitskaya K.V., upadyshev M.T., Petrova A.D., upadysheva G.Yu. Monitoring of harmful viruses in sour cherry and sweet cherry plantations in the Moscow Region. Pomiculture and Small Fruits Culture in Russia. 2016; 46: 227–231. (In Rus.)

15. Mikheev A.M., Revyakina N.T. Cherry. Moscow: Agropromizdat, 1991: 40. (In Rus.)

16. Mikheev A.M., Evstratov A.I., Simonov V.S. New promising cultivars of cherry and plum in the conditions of the Non-Chernozem Region. Pomiculture and Small Fruits Culture in Russia. 1997; 4: 14–19. (In Rus.)

17. Molkanova O.I., Koroleva O.V., Stakheeva T.S., Krakhmaleva I.L., Meleshchuk E.A. Improving the technology of clonal micropropagation of valuable fruit and berry crops for production conditions. Achievements of Science and Technology in Agro-Industrial Complex. 2018; 32 (9): 66–69. DOI: 10.24411/0235–2451–2018–10915 (In Rus.)

18. Orlova S.Yu. Biological features and breeding value of cherry cultivars in the conditions of the North-West of Russia. CSc (Bio) thesis. St. Petersburg, 2002: 20. (In Rus.)

19. Ostrikova O.V., Fedotova I.E., Kharkhardina E.L. Study of the reproductive potential of clonal cherry rootstocks during clonal micropropagation. Pomiculture and Small Fruits Culture in Russia. 2020. 59: 39–46. DOI: 10.31676/2073–4948–2019–59–39–46 (In Rus.)

20. Prikhod’ko Yu.N., Chirkov S.N., Metlitskaya K.V., Tsubera L.V. Prevalence of viral diseases of stone fruit crops in the European part of Russia. Agricultural Biology. 2008; 1: 26–32. (In Rus.)

21. Shevelukha V.S. Agricultural biotechnology and bioengineering. Textbook. Moscow: URSS, 2015: 715. (In Rus.)

22. Solonkin A.V. Cherry and plum breeding strategy to create cultivars cultivated using modern technologies in the Lower Volga Region. DSc (Ag) hesis. Volgograd, 2017: 349. (In Rus.)

23. Faustov V.V., Oleshko E.V., Zharkova I.V., Asadulaev Z.M., Sharafutdinov Kh.V., Ismail Kh. Clonal micropropagation of cherry. Izvestiya of Timiryazev Agricultural Academy. 1988; 5; 131–148. (In Rus.)

24. Sharafutdinov Kh.V. Sour cherry and sweet cherry seedlings. Effective methods for obtaining grafted plants. Monograph. Moscow: MESKh, 2020: 136. (In Rus.)

25. Shakhov V.V., Fedotova I.E., Tashmatova L.V., Matsneva O.V., Khromova T.M. Effect of the seasonal factor on the survival of sour cherry explants in in vitro culture. International Research Journal. 2020; 11–1 (101): 159–162. DOI: 10.23670/IRJ.2020.101.11.027 (In Rus.)

26. Cerović R., D. Ružić Micropropagation of Sour Cherry (Prunus cerasus L.) cv. Šumadinka. Plant Cell, Tissue and Organ Culture. 1987; 9: 151–157.

27. Dorić D., Ognjanov V., Barać G., Ljubojević M., Pranjić A., Dugalić K., Ercişli S. Use of In Vitro Propagation of ‘Oblačinska’ Sour Cherry in Rootstock Breeding. Turkish Journal of Biology. 2015; 39; 4: 8. [Electronic source]. URL: https://journals.tubitak.gov.tr/biology/vol39/iss4/8 DOI: 10.3906/biy-1412–85

28. Gashi A., Thomaj T., Mitrushi T., Kukali E. Optimization of Nutrient Media of In Vitro Propagation for Some Cherry Rootstocks (Prunus cerasus). Anglisticum Journal (IJLLIS). 2017; 6; 12: 54–61. DOI: 10.5281/zenodo.1134340

29. Singh S.R., Sundouri A.S., Sharma M.K., Srivastava K.K., Dar H.A. Proliferation and Rooting Efficiency Studies in Sour Cherry (Prunus cerasus) Using In Vitro Techniques. Journal of Horticultural Sciences. 2010; 5; 1: 48–52.

30. Tang H., Ren Z., Krczal G. Somatic Embryogenesis and Organogenesis from Immature Embryo Cotyledons of Three Sour Cherry Cultivars (Prunus cerasus L.). Scientia Horticulturae. 2000; 83: 109–126.