Effect of the drug Roncoleukin on morphometric and haematological parameters and phagocytic activity of arctic char neutrophils

Cultivated hydrobionts under intensive aquaculture conditions are exposed to stress factors associated with artificial habitats, increased planting density, periodic fishing, etc. At the same time, they experience a decrease in resistance and resilience and an increase in morbidity. A good solution to this problem is the use of immunomodulators. This article presents the results of a study of the effect on fish of the immunomodulator Roncoleukin, which is a dosage form of recombinant human interleukin‑2 (IL-2) isolated and purified from yeast cells (Saccharomyces cerevisiae). The subject of the study was the three-year-old Arctic char (Salvelinus alpinus). The evaluation was based on morphometric, hematological (erythro- and leukogram) and cytochemical (lysosomal cation test) indicators. The coefficient of variation of the size and weight parameters of the treated fish was low compared to the control group. This indicates uniform growth of fish fed Roncoleukin, which is a great advantage in fish farming and provides more efficient use of feed. Blood for analysis was taken from the tail vein of the fish in vivo. The results showed a positive effect of the drug on the activation of erythropoiesis and leukopoiesis. There was a significant increase in the proportion of mature segmented neutrophils (the main blood microphages belonging to the non-specific link of cellular immunity) in the leukocyte formula due to a decrease in lymphocytes (specific immunity). An increase in the content of non-enzymatic lysosomal cationic proteins (defensins) in blood neutrophils can indicate a high level of cellular protection of microphages (neutrophils). The results of the study showed a positive effect of the drug Roncoleukin, which can be recommended for use in the ferming of Arctic char.

1. Bessonov N.M. Privezentsev, Yu.A. Fishery hydrochemistry. Moscow, Russia: Agropromizdat, 1987:159. (In Russ.)

2. Velikanov V.I., Klyapnev A.V Comparison efficiency of application roncoleukinum and polyoxido-nium on physiological conditions and non-specific resistance of calves. Veterinarniy vrach. 2021;4:10–16. (In Russ.)

3. Egorova V.N., Gizinger O.A. Possibilities of pathogenetic therapy with interleukin 2 in animal husbandry (review). Glavniy zootekhnik. 2023;2:73–79. (In Russ.)

4. Egorova V.N., Romanova O.V., Belova L.M. Combined use of recombinant interlekin 2 and anthelmintic drugs for the treatment of helminthiasis of animals. Agrarian science. 2023;(4):38–50. (In Russ.) https://doi.org/10.32634/0869–8155–2023–369–4–38–50

5. Moiseev A.N., Saharova E.D., Egorova V.N., Ostrovskiy M.V., Romanova O.V., Grechuhin A.N., Nekrasov A.A., Varyukhin A.V., Baryshnikov P.I. Roncoleukin: application in farm animals. Sankt-Peterburg, Russian: Al’ter Ego, 2012:36. (In Russ.)

6. Pereryadkina S.P., Boldarev A.A., Kolesnikov P.V., Galchenko V.A. Search and development of a method of therapy for postpartum endometritis in cows. International Journal of Veterinary Medicine. 2020;(2):204–209. (In Russ.)

7. Pishchenko E.V. Haematology of freshwater fish: a textbook. Novosibirsk: Novosibirskiy gosudarstvenniy agrarniy universitet, 2002: 48. (In Russ.)

8. Pronina G.I. Norms of haematological, cytochemical and biochemical indices for health assessment of fish and river crayfish in aquaculture. Moscow, 2021. Approved by the NTS of the Department of Veterinary Medicine of the Ministry of Agriculture of the Russian Federation from 25.08.2021. (In Russ.)

9. Pronina G.I. The possibility to increase the immune resistance of hydrobionts in aquaculture. Izvestiya Orenburgskogo GAU. 2014;3:180–183. (In Russ.)

10. Pronina G.I., Koryagina N.Yu. Methodology of physiological and immunological assessment of hydrobionts: a textbook. Sankt-Peterburg, Russia: Lan’, 2017:96. (In Russ.)

11. Chagina E.A., Sgurskaya L.V., Silkin S.V., Medvedeva Yu.E., Shapovalov A.S. Effect of Ronkoleikin (IL 2) on the phagocytic link of immunity on the model of experimental peritonitis. Fundamental Research. 2004;4:89–90. (In Russ.)

12. Chagina E.A., Sgurskaya L.V., Silkin S.V., Medvedeva Yu.E., Shapova¬lov A.S. Clinical and experimental characteristics of the effect of ronkoleikin (IL 2) on the phagocytic reserve of neutrophils in patients with peritonitis. Fundamental Research. 2004;5:135–136. (In Russ.)

13. Gormaz J.G., Fry J.P., Erazo M., Love D.C. Public Health Perspectives on Aquaculture. Curr Environ Health Rep. 2014; 1(3):227–238.

14. Justiz-Vaillant A.A., Qurie A. Interleukin. StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. URL: https://www.ncbi.nlm.nih.gov/books/NBK499840/

15. Ul-Haq Z., Naz S., Mesaik M.A. Interleukin 4 receptor signaling and its binding mechanism: A therapeutic insight from inhibitors tool box. Cytokine & Growth Factor Reviews. 2016;32:3–15. https://doi.org/10.1016/j.cytogfr.2016.04.002