Study of the effi ciency of new nutrient media for the production of improved strawberry seedlings by in vitro
https://doi.org/10.26897/0021-342X-2022-5-21-34
Abstract
Current trends in the development of the horticulture industry have shown that in order to meet the needs of the Russian population for fruits and berries, it is necessary to expand the plantations of these crops. Clonal micropropagation is a modern method of mass, accelerated vegetative propagation of plants, which is widely used in the production of domestic planting material for garden plants. However, given that most of the nutrient media used today in the practice of clonal micropropagation were created in 70–80 years of XX century, there is a need to improve its optimal compos ition. In this regard, the purpose of this work was to study the effect of replacing inorganic salts of all microelements that are part of the nutrient medium with complexonates of microelements with a carboxyl-containing ligand – E DTA. A comparative evaluation of the studied nutrient media revealed a significant advantage in plant regeneration ability in all strawberry cultivars on modified nutrient medium No. 1, where the deviation of the multiplier coefficients of the experimental variants from the control was 0.6–1.8 units on the second passage and 0.9–2.9 units. on the third. At the stage of rhizogenesis, the experimental strawberry microplants were highly sensitive to the nutrient media, and the rooting of microrosettes exceeded that of the control by 6.2–25.0% on the 28th day of subcultivation.
About the Authors
A. N. Esaulko
Stavropol State Agrarian University
Russian Federation
Russian Federation
Aleksandr N. Esaulko, DSc (Ag), Professor of the Russian Academy of Sciences, Dean of the Faculties of Agrobiology and Land Resources, Ecology and Landscape Architecture
12 Zootekhnicheskiy Lane, Stavropol, 355017
phone: (962) 400–41–95
A. K. Radzhabov
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Agamagomed K. Radzhabov, DSc (Ag), Professor, Director of the Institute of Horticulture and Landscape Architecture
49 Timiryazevskaya Str., Moscow, 127434
T. S. Aysanov
Stavropol State Agrarian University
Russian Federation
Russian Federation
Timur S. Aysanov, PhD (Ag), Associate Professor of the Department of Production and Processing of Foodstuffs from Vegetable Raw Materials
12 Zootekhnicheskiy Lane, Stavropol, 355017
phone: (988) 629–63–77
V. Yu. Velichko
Stavropol State Agrarian University
Russian Federation
Russian Federation
Vitaliy Yu. Velichko, PhD (Bio), Head of the Laboratory of Agricultural Biotechnology
12 Zootekhnicheskiy Lane, Stavropol, 355017
phone: (918) 801–14–50
S. V. Akimova
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Svetlana V. Akimova, PhD (Ag), Associate Professor, Associate Professor of the Department of Fruit Growing, Viticulture and Winemaking
49 Timiryazevskaya Str., Moscow, 127434
E. A. Ninulina
Institute of Chemical Reagents and High Purity Chemical Substanc es
of National Research Center “Kurchatov Institute”; National Research Center “Kurchatov Institute”
Russian Federation
Russian Federation
Elena A. Nikulina, Deputy Head of the Laboratory of Custom Organic
Synthesis, Institute of Chemical Reagents and High Purity Chemical Substances
3 Bogorodsky Val Str., Moscow, 107076
A. E. Matsneva
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Anna E. Matsneva, Head of the Department of Biotechnology and Berry Crops of the Research and Production Centre for Horticulture and Vegetable Growing named after V.I. Edelstein
49 Timiryazevskaya Str., Moscow, 127434
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For citations:
Esaulko A.N., Radzhabov A.K., Aysanov T.S., Velichko V.Yu., Akimova S.V., Ninulina E.A., Matsneva A.E. Study of the effi ciency of new nutrient media for the production of improved strawberry seedlings by in vitro. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2022;(5):21-34. (In Russ.) https://doi.org/10.26897/0021-342X-2022-5-21-34
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