Genetic structure of west siberian meat sheep and kulunda fi ne-wool sheep by CAST, GDF9 AND KRT1.2 genes

The purpose of the study is to analyze the genotypic structure and population-genetic parameters of the West Siberian meat and Kulunda fine-wool breed of sheep by the CAST, GDF9 and KRT1.2 genes and blood groups. Molecularnogenetic and immunogenetic studies were carried out in the SRIAH (Siberian Research Institute of Animal Husbandry SFSCA RAS) biotechnology laboratory. PCR-RFLP analysis to determine genotypes was performed according to the described and approved methods on a C1000 “BioRad” amplifier; the results were visualized using an E-Box-CX5.TS-20.M gel documentation system. It was found that the frequency of genotypes and alleles of the CAST gene of West Siberian meat and Kulunda fine-wool breeds have no significant differences. In both breeds, CAST^MM was predominant, with a frequency of 69.0–75.0%, CAST^NN carriers being only 1.6–5.1%. The Kulunda fine-wool breed is characterized by a very high frequency of the GDF9^GG genotype 92.7%, which is higher than that of the West Siberian meat sheep by 26.3%. GDF9^AA genotype is extremely rare in breeds (0–5%). The GDF9^G allele in the compared breeds was found to range from 0.811–0.960 and the GDF9^A allele from 0.189–0.040. There are differences in the ratio of genotypes of the KRT1.2 gene in the compared breeds. In the Kulunda fine-wool breed, the vast majority of animals have homozygous genotype KRT1.2^MM (95.5%), whereas in the West Siberian meat breed such sheep constitute 32.8%. Consequently, the frequency of the KRT1.2^M allele is 0.565–0.978 and the KRT1.2N allele is 0.435–0.022. The genetic balance in the studied genes is not disturbed, χ2 = 0,033–1,025. The index of genetic similarity between breeds, calculated on the basis of the frequencies of genotypes and blood groups is 0.901 ± 0.028 and 0.833 ± 0.024. Population and genetic characteristics are practically identical in the compared breeds, except for gene homozygosity (C_a), which is higher in the Kulunda fine-wool sheep breed by 24.3% for the GDF9 gene and KRT1.2 by 29.1%, compared to the West Siberian meat sheep breed. The number of effective alleles is insignificant (1.04–1.46). The genetic variability (V) by individual genes in the breeds varies from 5.0 to 49.6%. The negative value of the F_is coefficient indicates the absence of inbreeding in sheep the studied breeds.

1. Aboneev V.V., Kolosov Ju.A. O problemah sohranenija plemennyh resursov ovcevodstva Rossii [On the problems of preserving the breeding resources of sheep breeding in Russia] // Ovcy, kozy, sherstyanoe delo. 2020; 1: 43–45. (in Rus.)

2. Aboneev V.V., Kolosov Ju.A., Lagoda A. K voprosu o plemennyh resursah ovcevodstva [On the issue of breeding resources of sheep breeding] // Prioritetnye napravleniya razvitiya sel’skohozyaistvennoi nauki i praktiki v APK. Materialy vserossiiskoi (nacional’noi) nauchno-prakticheskoi konferencii. V 3-h tomah. pos. Persianovskii, 2021: 177–180. (in Rus.)

3. Gorlov I.F., Slozhenkina M.I., Kolosov Ju.A., Shirokova N.V. Geneticheskaya struktura stada po genam GDF9, GH u ovec volgogradskoi i edil’baevskoi porod [Genetic structure of the herd according to the GDF9, GH genes in sheep of the Volgograd and Edilbaev breeds] // Agrarno-pishevye innovacii. 2021; 14; 2: 51–59. (in Rus.)

4. Erohin A.I., Karasev E.A., Erohin S.A. Sostoyanie, dinamika i tendencii v razvitii ovcevodstva v mire i v Rossii [Status, dynamics and trends in the development of sheep breeding in the world and in Russia] // Ovcy, kozy, sherstyanoe delo. 2019; 3: 3–7. (in Rus.)

5. Katamanov S.G., Ul’janov A.N., Kulikova A.Ja.,. Aboneev V.V, Sel’kin I.I., Afanas’eva A.I., Katamanov Ju.G., Loboda N.D.. Moroz V.A., Truhachev V.I., Katamanov A.S., Chebotaev A.N., Chmyrev M.A. Zapadno-Sibirskaya myasnaya poroda ovec [West Siberian meat breed of sheep] // Ovcy, kozy, sherstyanoe delo. 2012; 3: 6–12. (in Rus.)

6. Kuznecov V.M. Osnovy nauchnyh issledovanii v zhivotnovodstve.[Fundamentals of scientific research in animal husbandry] Kirov, Zonal’nyi NIISKH Severo-Vostoka, 2006: 568. (in Rus.)

7. Lushnikov V.P., Fetisova T.O., Selionova M.I., Chizhova L.N., Surzhikova E.S. Polimorfizm genov somatotropina (GH), kal’pastatina (CAST), differencial’nogo faktora rosta (GDF9) u ovec tatarstanskoi porody [Polymorphism of somatotropin (GH), calpastatin (CAST), differential growth factor (GDF9) genes in sheep of the Tatarstan breed] // Ovcy, kozy, sherstyanoe delo. 2020; 1: 2–3. (in Rus.).

8. Lushnikov V.P., Fetisova T.Ju., Stril’chuk A.A. Polimorfizm gena CAST u ovec tatarstanskoi i edil’baevskoi porod [Polymorphism of the CAST gene in sheep of the Tatarstan and Edilbaev breeds] // Ovcy, kozy, sherstyanoe delo. 2020; 2: 9–11. (in Rus.)

9. Moroz V.A., Katamanov S.G., Kotomanov Ju.G., Bida P.S., Truhachev V.I., Kesler N.F., Storozhuk S.I., Rybalko I.V., Sel’kin I.I. Novaya tonkorunnaya poroda ovec – Kulundinskaya [New fine-wool breed of sheep – Kulunda] // Ovcy, kozy, sherstyanoe delo. 2008; 3: 6–9. (in Rus.)

10. Ozderimov A.A., Chizhova L.N., Hozhokov A.A., Surzhikova E.S., Dogeev G.D., Abdulmagomedov S.Sh. Polimorfizm genov CAST, GH, GDF9 dagestanskoi gornoi porody [Polymorphism of the CAST, GH, GDF9 genes of the Dagestan breed] // Jug Rossii: ekologiya, razvitie. 2021; 16; 2: 39–44. (in Rus.)

11. Pogodaev V.A., Kononova L.V., Aduchiev B.K. Polimorfizm genov kal’pastatina i somatotropina u ovec kalmyckoi kurdyuchnoi porody i pomesei (1/2 kalmyckaya kurdyuchnaya + 1/2 dorper) [Polymorphism of calpastatin and somatotropin genes in sheep of the Kalmyk fat-tailed breed and crossbreeds (1/2 Kalmyk fat-tailed + 1/2 Dorper)] // Vestnik Ul’yanovskoi gosudarstvennoi sel’skohozjajstvennoi akademii. 2019; 3 (47): 141–145. (in Rus.)

12. Selionova M.I., Evlagina D.D., Svetlichnyj S.I. Polimorfizm gena GDF9 i ego svyaz’ s molochnoi produktivnost’yu ovec porody Lakon [Polymorphism of the GDF9 gene and its relationship with the milk productivity of sheep of the Lacon breed] // Materialy 3-ei Mezhdunarodnoi nauchno-prakticheskoi konferencii «Molekulyarno-geneticheskie tehnologii analiza ekspressii genov produktivnosti i ustoichivosti k zabolevaniyam zhivotnykh» v ramkakh goda nauki i tekhnologii Rossiiskoi Federacii po tematike «Genetika i kachestvo zhizni» Moskva, 2021: 396–403. (in Rus.)

13. Selionova M.I., Chizhova L.N., Surzhikova E.S., Podkorytov N.A., Podkorytov A.T. Polimorfizm genov CAST, GH, GDF9 ovec gorno-altajskoj porody [Polymorphism of CAST, GH, GDF9 genes in Mountain-Altai sheep] // Sibirskii vestnik sel’skohozyaistvennoi nauki. 2020; 50; 1: 92–100. (in Rus.)

14. Sel’kin I.I., Katamanov A.S. Zhivaja massa, nastrig i svoistva shersti novoi Kulundinskoi tonkorunnoi porody ovec [Live weight, shearing and wool properties of the new Kulunda fine-wool breed of sheep] // Ovcy, kozy, sherstyanoe delo. 2009; 1: 8–10. (in Rus.)

15. Senina R.Ju., Kalashnikova L.A., Lushnikov V.P., Pavlov M.B. Polimorfizm gena KRT1.2 u otechestvennykh porod ovec [Polymorphism of the KRT1.2 gene in domestic breeds of sheep] // Ovcy, kozy, sherstyanoe delo. 2018; 3: 20–23. (in Rus.)

16. Hamiruev T.N. Immunogeneticheskaya kharakteristika ovec zabaikal’skoi porody raznogo napravleniya produktivnosti [Immunogenetic characteristics of sheep of the Transbaikal breed of different directions of productivity] // Rossiiskaya sel’skohozyaistvennaya nauka. 2019; 2: 53–55. (in Rus.)

17. Chizhova L.N., Aboneev V.V., Aboneev D.V., Shumaenko S.N. Immunogeneticheskaja sochetaemost’ roditel’skih par i morfostrukturnye pokazateli placenty ovec [Immunogenetic compatibility of parental pairs and morphostructural parameters of sheep placenta] // Novye podkhody, principy i mekhanizmy povysheniya effektivnosti proizvodstva i pererabotki sel’skokhozyaistvennoi produkcii: materialy Mezhdunarodnoi nauchno- prakticheskoi konferencii. Pod obshhej redakciej I.F. Gorlova; GNU Povolzhskij NII proizvodstva i pererabotki myasomolochnoy produkcii Rossel’hozakademii, Volgogradskii gosudarstvennyi tehnicheskii universitet, 2014: 144–147. (in Rus.)

18. Chizhova L.N., Surzhikova E.S., Luciva E.D., Efimova N.I. Polimorfizm gena CAST, osobennosti zhirnokislotnogo sostava lipidov krovi ovec raznyh genotipov v ontogeneze [Polymorphism of the CAST gene, features of the fatty acid composition of blood lipids in sheep of different genotypes in ontogenesis] // Vestnik Kurskoi gosudarstvennoi sel’skokhozyaistvennoi akademii. 2020; 6: 47–51. (in Rus.)

19. Abbott J.K.R., Hickford J.G.H., Summer R.M.W. Gene markers for wool fibre traits // Proceedings of the New Zealand Society of Animal Production. 2006, 66: 139–144.

20. Al-Khuzai F.L.J. J., Ahmed R. Polymorphism of GDF9 (exon-1) gene and its association with milk production and prolificacy of Awassi sheep // Plant Archives. 2019; 19; 2: 4037–4040.

21. Getmantseva L Bakoev N., Shirokova N., Kolosova M., Bakoev S., Kolosov A. et. al. Effect of the gene GDF9 on weight of lambs at birth // Bulgarian Journal of Agricultural Science. 2019; 25; 1: 153–157.

22. Gong H., Zhou H., Rachel H.J. Forrest, Shaobin Li, Jiqing Wang, Jolon M. Dyer, Yuzhu Luo, and Jon G.H. Hickford // Wool keratin-associated protein genes in sheep-A Review // Genes. 2016; 7; 24: 1–16.

23. Hanrahan J.P., Gregan S.M., Milsant P., et. al. Mutations in the genes for oocytederived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and belclare ship (Ovis aries) // Biology Of Reproduction. 2004; 70; 4: 900–909.

24. Khederzadeh S. Genetic diversity of myostatin and calpastatin genes in Zandi sheep / S. Khederzadeh, M. Iranmanesh, R. Motamedi-Mojdehi // Journal of Livestock Science and Technologies. 2016; 4(1): 45–52.

25. Kolosov Yu.A., Getmantseva L, V., Shirokova N.V. et.al. Polymorphism of the GDF9 Gene in Russian Sheep Breeds // J. Cytol Histol. 2015; 6: 305.

26. Kumar R., Meena A.S., Kumari R., Jyotsana B., Prince L.L.L. and Kumar S. Polymorphism of KRT 1.2 and KAP 1.3 genes in Indian sheep breeds // Indian Journal of Small Ruminants. 2016; 22(1): 28–31.

27. Rogers G., Hickford J., Bickerstaffe R.A. Potential QTL for wool strength located on ovine chromosome 11 // Proceedings of the World Congress on Gen etics Applied to Livestock Production. 1994; 21: 291–294.

28. Selionova M.I., Podkorytov N.A. Polymorphism of GDF9 in sheep of Prikatun type of Altai Mountains breedand its correlations with indices of meat rate productivity // Theory and Practice of Meat Processing. 2021; 6; 1: 4–9.

29. Kolosov Yu.A., Gorlov I.F., Kolosov A.Yu., Shirokova N.V., Kulikova A.Ya., Kolosova M.A., Slozhenkina M.I., Vorontsova E.S. and Kolosova N.N. Determination of CAST gene polymorphism in sheep of the Volgograd breed // IV internationalconference on agribusiness, environmental engineering and biotechnologies – Agritech-iv – 2020 IOP conference series: earth and environmental science, Krasnoyarsk. 2021: 1–7.