Initiation of horticultural crop explants in vitro
https://doi.org/10.26897/0021-342X-2022-4-71-81
Abstract
The stage of introduction into sterile culture plays an important role in clonal micropropagation. The purpose of these studies was to find the optimal sterilizing agent at the stage of introducing blue honeysuckle, red raspberry, garden strawberry, steppe cherry, house plum into in vitro culture, as well as the optimal nutrient medium at this stage for blue honeysuckle and red raspberry. As sterilizing agents with optimal exposure, the following were used: 48% ethyl alcohol (control) 8–10 min., 33% perhydrol 6–8 min., 6% chlorhexidine 8–10 min., 0.1% sublimate 1–2 min., 10.0% Domestos 6–7 min., Amway pursue 10–12 min. Murashige-Skooga modified (NH4 content reduced at 15%) and Woody Plant Medium nutrient media were used to identify the optimal media for blue honeysuckle, and Anderson and Quorin-Leporrier media – for raspberries. All nutrient media had a half dose of macro- and microsalts. The control medium was traditional Murashige-Skooga. The results of the experiment showed that 33.0% perhydrol is the most effective sterilizing agent for processing the initial plant material of all horticultural crops when initiating explants in vitro, since it provided a significantly high yield of sterile apexes of 63.0% on average. A significantly high result was also obtained when using 6% chlorhexidine – 51.9% with 39.1% in control (48% ethyl alcohol). Treatment of plant material with 33.0% perhydrol ensured the absence of vitrified (glassy) explants in all studied cultures, as well as chlorotic explants in cherries and plums. Significantly better results of the survival rate of blue honeysuckle apexes were obtained on the nutrient medium Woody Plant Medium – 76.7%. Cultivation of raspberry apexes on the Kvorin-Leporie nutrient medium significantly increased their survival rate to 56.7% of red raspberry and 36.7% of remontant raspberry.
About the Authors
M. G. Markova
«Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences»
Russian Federation
Russian Federation
Marina G. Markova, research, Federal State Budgetary Institution of Science
426067, Udmurt Republic, Izhevsk, st. T. Baramzinoj, 34
(919) 912–05–07
E. N. Somova
«Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences»
Russian Federation
Russian Federation
Elena N. Somova, research Federal State Budgetary Institution of Science
426067, Udmurt Republic, Izhevsk, st. T. Baramzinoj, 34
(950) 175–46–27
References
1. Bessonova V.A., CHerepanova O.E. Vvedenie v kul’turu in vitro Ginkgo biloba (Linnaeus,1771) [Introduction to in vitro culture of Ginkgo biloba (Linnaeus, 1771)] Agrarnyj vestnik Urala. 2020; 12 (203): 43–49. (In Rus.)
2. Vershinina O.V., B”yadovskij, I.A. Osobennosti razvitiya eksplantov rubus idaeus L. i ribes nigrum L. perspektivnyh sortov selekcii FGBNU FNC sadovodstva na etape vvedeniya v kul’turu in vitro[Features of the development of explants rubus idaeus L. and ribes nigrum L. of promising varieties of breeding of the Federal State Budgetary Institution of Horticulture at the stage of introduction into culture in vitro] Plodovodstvo i yagodovodstvo Rossii. 2020; 63: 44–52. (In Rus.)
3. Vinter M.A., Fedorovich S.V., Thamokova I.H. Effektivnost’ antiseptika “biopak” pri sanacii eksplantov v hode klonal’nogo mikrorazmnozheniya plodovyh kul’tur [The effectiveness of the antiseptic “biopak” in the rehabilitation of explants during clonal micropropagation of fruit crops] Politematicheskij setevoj elektronnyj nauchnyj zhurnal kubanskogo gosudarstvennogo agrarnogo universiteta. 2020; 162: 105–113. (In Rus.)
4. Vysockij V.A. Podhody k prognozirovaniyu konechnogo vyhoda rastenij pri klonal’nom mikrorazmnozhenii plodovyh i yagodnyh kul’tur [Approaches to predicting the final yield of plants during clonal micropropagation of fruit and berry crops]. Selekciya i sortorazvedenie sadovyh kul’tur. 2019; 1(6): 24–26. (In Rus.)
5. GOST R54051–2010 Plodovye i yagodnye kultury sterilnye kultury i adaptirovannye mikrorasteniya tekhnicheskie usloviya [Fruit and berry crops. Sterile cultures and adapted microplants. Specifications].2010; 15 s. (In Rus.)
6. Dospekhov B.A. Metodika polevogo opyta (s osnovami statisticheskoj-obrabotki rezultatov issledovanij) [Field experience methodology: (with the basics of statistical processing of research results]: uchebnik dlya studentov vysshih-selskohozyajstvennyh uchebnyh zavedenij po agronomicheskim specialnostyam / izd 6-e ster perepech s 5-go izd 1985g. Moscow: Alliance, 2011. 350 s. (In Rus.)
7. Eremin G.V., Eremin V.G. Ispol’zovanie genofonda dikorastushchih vidov roda Prunus L. v selekcii klonovyh podvoev kostochkovyh kul’tur [The use of the gene pool of wild species of the genus Prunus L. in the selection of clone rootstocks of stone crops]. Trudy po prikladnoj botanike, genetike i selekcii. 2015; 4(176): 416–428. (In Rus.)
8. Kolbanova E.V., Semenas S.E. Vvedenie v kul’turu in vitro sortov zhimolosti sinej (Lonicera caerulea l. Var. Kamtschatica) [Introduction to in vitro culture of varieties of blue honeysuckle (Lonicera caerulea l. Var. Kamtschatica)]. Plodovodstvo. sbornik nauchnyh trudov. Izdatel’stvo: Respublikanskoe unitarnoe predpriyatie “Izdatel’skij dom “Belorusskaya nauka”. Minsk: 2019: 162–168 (In Rus.)
9. Kulikova E.I., Makarov S.S., Kuznecova I.B., CHudeckij A.I. Osobennosti kul’tivirovaniya rossijskih i zarubezhnyh sortov zhimolosti s”edobnoj (Lonicera edulis turcz.) in vitro [Features of cultivation of Russian and foreign varieties of edible honeysuckle (Lonicera edulis turcz.) in vitro]. Tekhnika i tekhnologiya pishchevyh proizvodstv. 2020; 4 (51): 712–722. (In Rus.)
10. Makarov S.S., Kuznecova I.B., Smirnov V.S. Vliyanie sposobov sterilizacii i tipov eksplantov zhimolosti sinej na ih zhiznesposobnost’ v usloviyah in vitro [Influence of sterilization methods and types of blue honeysuckle explants on their viability in vitro] Lesohozyajstvennaya informaciya. 2018; 2: 96–101. (In Rus.)
11. Markova M.G., Somova E.N. Regeneracionnaya sposobnost’ Cerasus fruticosa i Prunus domestica v kul’ture in vitro [Regenerative capacity of Cerasus fruticosa and Prunus domestica in in vitro culture]. Agrarnyj Vestnik Urala. 2021; 6 (209): 43–52. (In Rus.)
12. Markova M.G., Somova E.N. Klonal’noe mikrorazmnozhenie yagodnyh kul’tur [Clonal micro-propagation of berry crops] Izhevsk: Izd-vo «Alkid». 2020: 102. (In Rus.)
13. Macneva O.V., Tashmatova L.V., Hromova T.M., SHahov V.V. Vvedenie sortov zemlyaniki v kul’turu in vitro [Introduction of strawberry varieties into culture in vitro]. Plodovodstvo i yagodovodstvo Rossii. 2019; 56: 28–34. (In Rus.)
14. Upadyshev M.T. Tekhnologiya polucheniya ozdorovlennogo ot virusov posadochnogo materialy plodovyh i yagodnyh kul’tur [Technology for obtaining virus-cured planting materials of fruit and berry crops]: metodicheskie ukazaniya – Moskva: Rosinformagrotekh. 2013: 91. (In Rus.)
15. Shahov V.V., Fedotova I.E., Tashmatova L.V., Macneva O.V., Hromova T.M. Vliyaniya sezonnogo faktora na prizhivaemost’ eksplantov vishni obyknovennoj v kul’ture in vitro [The influence of the seasonal factor on the survival rate of explants of the common cherry in culture in vitro] Mezhdunarodnyj nauchno-issledovatel’skij zhurnal. 2020; 11–1(101): 159–162. (In Rus.)
16. Shahov V.V., Tashmatova L.V., Macneva O.V., Hromova T.M. Effektivnost’ sterilizuyushchih agentov pri vvedenii sortov vishni v kul’turu in vitro [The effectiveness of sterilizing agents when introducing cherry varieties into culture in vitro] Sovremennoe sadovodstvo. 2018; 4 (28): 32–37 (In Rus.)
17. Yancheva S., Marchev R., Yaneva V.et al. In vitro propagation of grape cultivars and rootstocks for production of pre-basic planting material. Bulgarian Journal of Agricultural Science. 2018;5 (24): 801–806. (In Bolgariya)
18. Ghasheem N. AL, Stănică F., Peticilă A.G., Venat O. In vitro effect of various sterilization techniques on peach (Prunus persica (L.) Batsch) explants. Scientifc Papers Series B, Horticulture. 2018; LXII: 227–234.
Review
For citations:
Markova M.G., Somova E.N. Initiation of horticultural crop explants in vitro. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2022;(4):71-81. (In Russ.) https://doi.org/10.26897/0021-342X-2022-4-71-81
Views:
158
Email this article 