Cryoprotectant evaluation in spring rape under model low-temperature stress
https://doi.org/10.26897/0021-342X-2025-4-90-100
Abstract
This study investigated the effects of cryoprotectants with various compositions on the lowtemperature stress resistance of spring rape (Oredezh 6 variety) in a model experiment. The cryoprotectants used were preparations containing a complex of chemical substances, including polysaccharides, amino acids, glycol, and chelated microelements. The experimental design included the following treatments: 1) control (water), no freezing; 2) control (water), freezing; 3) cryoprotector LP 8; and 4) cryoprotector LP 10. Twenty-four hours prior to freezing, spring rape plants in the 2–3 leaf phase were treated with cryoprotectors. Plants were frozen twice in laboratory freezers at –10°C for 16-hour periods, with an 8-hour interval between freezing cycles. Plant weight was measured, leaf area was determined using the Saporeo program, sugar content was assessed via refractometry, electrolyte leakage was measured using a conductometer, and chloroplast activity was assessed using a portable N-tester. Following the freezing treatments, the effects of cryoprotectors were evaluated. Results showed that plants treated with cryoprotectors LP 8 and LP 10 exhibited increased resistance to low-temperature stress. This was evidenced by average increases of 26.8% in plant weight, 19.1% in leaf area, a two-fold increase in sugar content, a 6.7% increase in membrane stability index, and an 18% increase in photosynthetic activity compared to the freezing control (water). These findings suggest avenues for further research in this area.
About the Authors
V. A. Krylov
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy; JSC Firma Avgust
Russian Federation
Russian Federation
Vadim A. Krylov, CSc (Bio), Associate Professor at the Department of Agronomic, Biological Chemistry and Radiology; Head of the Plant Nutrition and Growth Regulation Group
49 Timiryazevskaya St., Moscow, 127550
V. A. Bolshov
JSC Firma Avgust
Russian Federation
Russian Federation
Aleksandr V. Bolshov, CSc (Bio), Head of the Group for Development of Preparations for Private Farms
6 Tsandera St., Moscow, 129515
T. E. Efreytorova
JSC Firma Avgust
Russian Federation
Russian Federation
Tatiana E. Efreytorova, CSc (Ag), Senior Research Associate at the Herbicide Group
Tsandera St., Moscow, 129515
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Supplementary files
Review
For citations:
Krylov V.A., Bolshov V.A., Efreytorova T.E. Cryoprotectant evaluation in spring rape under model low-temperature stress. IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY. 2025;(4):90-100. (In Russ.) https://doi.org/10.26897/0021-342X-2025-4-90-100
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