izvestiiatimacadИзвестия Тимирязевской сельскохозяйственной академииIZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY0021-342XФГБОУ ВО РГАУ-МСХА имени К.А. Тимирязева10.26897/0021-342X-2023-2-95-108izvestiiatimacad-444Research ArticleЗЕМЛЕДЕЛИЕ, РАСТЕНИЕВОДСТВО, ЗАЩИТА РАСТЕНИЙAGRONOMY, CROP PRODUCTION, PLANT PROTECTIONПервое сообщение о Cephalotrichum asperulum как патогене картофеля в РоссииFirst report on Cephalotrichum Asperulum As A Potato Pathogen In RussiaТучковИ. В.TuchkovI. V.

Тучков Иван Валерьевич, студент бакалавриата кафедры защиты растений

127550, г. Москва, ул. Тимиря­зевская, 49

Ivan V. Tuchkov, BSc student, Department of Plant Protection

49, Timiryazevskaya Str., Moscow, 127434

tuchkov_2002@mail.ruТаракановР. И.TarakanovR. I.

Тараканов Рашит Ислямович, аспирант кафедры защиты растений

127550, г. Москва, ул. Тимиря­зевская, 49

Rashit I. Tarakanov, post-graduate student, Department of Plant Protection

49, Timiryazevskaya Str., Moscow, 127434

tarakanov.rashit@mail.ruБелошапкинаО. О.BeloshapkinaO. O.

Белошапкина Ольга Олеговна, доктор сельскохозяйственных наук, профессор, профессор кафедры защиты растений

127550, г. Москва, ул. Тимиря­зевская, 49

Olga O. Beloshapkina, DSc (Ag), Professor, Department of Plant Protection

49, Timiryazevskaya Str., Moscow, 127434

beloshapkina58@mail.ruДжалиловФ. С.-У.DzhalilovF. S.-U.

Джалилов Февзи Сеид-Умерович, доктор биологических наук, профессор, заведующий кафедрой защиты растений

127550, г. Москва, ул. Тимиря­зевская, 49

Fevzi S.-U. Dzhalilov, DSc (Bio), Professor, Head of Department of Plant Protection

49, Timiryazevskaya Str., Moscow, 127434

dzhalilov@rgau-msha.ruРоссийский государственный аграрный университет – МСХА имени К.А. ТимирязеваРоссияRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian Federation2023120820230295108Copyright © Тучков И.В., Тараканов Р.И., Белошапкина О.О., Джалилов Ф.С., 20232023Тучков И.В., Тараканов Р.И., Белошапкина О.О., Джалилов Ф.С.Tuchkov I.V., Tarakanov R.I., Beloshapkina O.O., Dzhalilov F.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiia.timacad.ru/jour/article/view/444

При проведении фитосанитарной экспертизы в партии хранящегося картофеля сорта Жуковский ранний в Орловской области России обнаружены клубни с признаками поражений в виде локального потемнения мякоти без ее явного размягчения и коремии гриба на поверхности. Проведено выделение в чистую культуру патогена, и у выделенного изолята описаны морфологические особенности структур конидиального спороношения; приведены усредненные размеры вегетативного мицелия, конидий, конидиеносцев и коремий. Проверка патогенности изолята на листьях и ломтиках клубней показала наличие симптомов в виде локальных хлорозов и некрозов. Последующая идентификация изолята методами ПЦР и секвенирования показала, что анализируемый изолят наиболее близок к роду Cephalotrichum, виду C. asperulum. Полученная последовательность была депонирована в GenBank (регистрационный № ON364353). Несмотря на то, что выделенные грибы упоминаются в литературе как эндофитные, в данных исследованиях приоритетно показано их паразитическое воздействие на растение картофеля с определенной симптоматикой. Предположительно это первое сообщение о том, что C. asperulum вызывает локальный некроз листьев и клубней картофеля при хранении в России.

During the phytosanitary inspection of a lot of stored potatoes of cv. Zhukovskiy ranniy in the Orel region of Russia, tubers showed signs of lesions in the form of local darkening of the flesh without apparent softening and fungal structures on the surface (coremia). A pure culture of the pathogen was isolated and the morphological characteristics of the conidial sporulation structures in the extracted isolate were described; the average sizes of vegetative mycelium, conidia, conidiophores and coremia were given. Pathogenicity testing of the isolate on leaves and tuber discs showed symptoms in the form of localised chlorosis and necrosis. Subsequent identification of the isolate by PCR and sequencing showed that the isolate analysed was most closely related to the species Cephalotrichum asperulum of the genus Cephalotrichum. The resulting sequence was deposited in GenBank (accession number ON364353). Although these fungi are described in the literature as endophytic, this study emphasises their parasitic effect on the potato plant with certain semeiologies. This is probably the first report of C. asperulum causing local necrosis of potato leaves and tubers during storage in Russia.

Сephalotrichumгрибыпатогены картофеляэндофитыпервое обнаружениеискусственное заражениеCephalotrichumfungipathogen of potatoendophytethe first detectionartificial infectionРабота выполнена при поддержке Минобрнауки России в рамках соглашения № 075–15–2022–317 от 20 апреля 2022 г. о предоставлении гранта в форме субсидий из федерального бюджета на осуществление государственной поддержки создания и развития научного центра мирового уровня «Агротехнологии будущего»ReferencesFAOSTAT. Food Balance Sheet. Available online. – URL: http://www.fao.org/faostat/en/#data/FBS (accessed: on 2 Jule 2022).FAOSTAT. Food Balance Sheet. Available online: http://www.fao.org/faostat/en/#data/FBS (Access date: 02.06.2022).Voronina M.V., Lukianova A.A., Shneider M.M., Korzhenkov A.A., Toschakov S.V., Miroshnikov K.A., Vasiliev D.M. and Ignatov A.N. First Report of Pectobacterium Polaris Causing Soft Rot and Blackleg of Potato in Russia // Plant. – Dis. 2021. – № 105. – С. 6. https://doi.org/10.1094/PDIS-09–20–1864-PDN.Voronina M.V., Lukianova A.A., Shneider M.M., Korzhenkov A.A., Toschakov S.V., Miroshnikov K.A., Vasiliev D.M. and Ignatov A.N. First Report of Pectobacterium Polaris Causing Soft Rot and Blackleg of Potato in Russia. Plant Dis. 2021; 105; 6. https://doi.org/10.1094/PDIS-09–20–1864-PDNVoronina M.V., Kabanova A.P., Shneider M.M., Korzhenkov A.A., Toschakov S.V., Miroshnikov K.K., Miroshnikov K.A. and Ignatov A.N. First Report of Pectobacterium Carotovorum Subsp. Brasiliense Causing Blackleg and Stem Rot Disease of Potato in Russia // Plant Dis. – 2019. – № 103. – С. 2. https://doi.org/10.1094/PDIS-03–18–0456-PDN.Voronina M.V., Kabanova A.P., Shneider M.M., Korzhenkov A A., Toschakov S.V., Miroshnikov K.K., Miroshnikov K.A. and Ignatov A.N. First Report of Pectobacterium Carotovorum Subsp. Brasiliense Causing Blackleg and Stem Rot Disease of Potato in Russia. Plant Dis. 2019; 103; 2. https://doi.org/10.1094/PDIS-03–18–0456-PDNIgnatov A.N., Spechenkova N.A., Taliansky M. and Kornev K.P. First Report of Clavibacter michiganensis subsp. michiganensis Infecting Potato in Russia // Plant Dis. – 2019. – № 103 (1). https://doi.org/10.1094/PDIS-04–18–0691-PDN.Ignatov A.N., Spechenkova N.A., Taliansky M. and Kornev K.P. First Report of Clavibacter michiganensis subsp. michiganensis Infecting Potato in Russia. Plant Dis. 2019; 103; 1. https://doi.org/10.1094/PDIS-04–18–0691-PDNBelov G.L., Belosokhov A.F., Kutuzova I.A., Statsyuk N.V., Chudinova E.M., Alexandrova A.V., Kokaeva L.Y., Elansky S.N. Colletotrichum Coccodes in Potato and Tomato Leaves in Russia // J Plant Dis Prot. – 2018. – № 125. – Рр. 311–317. https://doi.org/10.1007/s41348–017–0138–0.Belov G.L.; Belosokhov A.F., Kutuzova I.A., Statsyuk N.V., Chudinova E.M., Alexandrova A.V., Kokaeva L.Y., Elansky S.N. Colletotrichum Coccodes in Potato and Tomato Leaves in Russia. J Plant Dis Prot. 2018; 125: 311–317. https://doi.org/10.1007/s41348–017–0138–0Chudinova E.M. and Elansky S.N. First report of Septotinia populiperda on potato tubers in Russia // J Plant Pathol. – 2021. – № 103. – Р. 665. http://dx.doi.org/10.1007/s42161–021–00751–2.Chudinova E.M. and Elansky S.N. First report of Septotinia populiperda on potato tubers in Russia. J Plant Pathol. 2021; 103; 665. http://dx.doi.org/10.1007/s42161–021–00751–2Girsova N., Bottner K.D., Mozhaeva K.A., Kastalyeva T.B., Owens R.A. and Lee I. – M. Molecular Detection and Identification of Group 16SrI and 16Sr XII Phytoplasmas Associated with Diseased Potatoes in Russia // Plant Dis. – 2008. – № 92. – Р. 4. https://doi.org/10.1094/PDIS-92–4–0654A.Girsova N., Bottner K.D., Mozhaeva K.A., Kastalyeva T.B., Owens R.A. and Lee I. – M. Molecular Detection and Identification of Group 16SrI and 16SrXII Phytoplasmas Associated with Diseased Potatoes in Russia. Plant Dis. 2008; 92; 4. https://doi.org/10.1094/PDIS-92–4–0654AOwens R.A., Girsova N.V., Kromina K A., Lee I.M., Mozhaeva K.A. and Kastalyeva T.B. Russian Isolates of Potato spindle tuber viroid Exhibit Low Sequence Diversity // Plant Dis. – 2009. – 93. – 7. https://doi.org/10.1094/PDIS-93–7–0752.Owens R.A., Girsova N.V., Kromina K.A., Lee I.M., Mozhaeva K.A. and Kastalyeva T.B. Russian Isolates of Potato spindle tuber viroid Exhibit Low Sequence Diversity. Plant Dis. 2009; 93; 7. https://doi.org/10.1094/PDIS-93–7–0752Carroll G.FungalEndophytesinStems andLeaves: FromLatentPathogentoMutualistic Symbiont // Ecology. – 1988. – № 69 (1). – Рр. 2–9. https://doi.org/10.2307/1943154.Carroll G. Fungal Endophytes in Stems and Leaves: From Latent Pathogen to Mutualistic Symbiont. Ecology. 1988; 69;1: 2–9. https://doi.org/10.2307/1943154Redman R.S., Freeman S., Clifton D.R., Morrel J., Brown G., Rodriguez R.J. Biochemical Analysis of Plant Protection Afforded by a Nonpathogenic Endophytic Mutant of Colletotrichum Magna // Plant Physiol. – 1999. – № 119. – Рр. 795–804. DOI:10.1104/pp.119.2.795.Redman R.S., Freeman S., Clifton D.R., Morrel J., Brown G., Rodriguez R.J. Biochemical Analysis of Plant Protection Afforded by a Nonpathogenic Endophytic Mutant of Colletotrichum Magna. Plant Physiol. 1999; 119: 795–804, doi:10.1104/pp.119.2.795Tadych M., White J. Endophytic Microbes // In Reference Module in Life Sciences. – 2019. – Рp. 123–136.Tadych M., White J. Endophytic Microbes. In Reference Module in Life Sciences. 2019: 123–136. ISBN978–0–12–811737–8Hardoim P.R., van Overbeek L.S., Berg G., Pirttilä A.M., Compant S., Campisano A., Döring M., Sessitsch A. The Hidden World within Plants: Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes // Microbiol Mol Biol Rev. – 2015. – № 79. – Рр. 293–320. https://doi.org/10.1128/MMBR.00050–14.Hardoim P.R., van Overbeek L.S., Berg G., Pirttilä A.M., Compant S., Campisano A., Döring M., Sessitsch A. The Hidden World within Plants: Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes. Microbiol Mol Biol Rev. 2015; 79: 293–320. https://doi.org/10.1128/MMBR.00050–14Bais H.P., Weir T.L., Perry L.G., Gilroy S., Vivanco J.M. The Role of Root Exudatesin Rhizosphere Interactions with Plants and Other Organisms//Annu Rev Plant Biol. – 2006. – № 57. – Рр. 233–266. – URL: DOI:10.1146/annurev.arplant.57.032905.105159.Bais H.P., Weir T.L., Perry L.G., Gilroy S., Vivanco J.M. The Role of Root Exudates in Rhizosphere Interactions with Plants and Other Organisms. Annu Rev Plant Biol. 2006; 57: 233–266. DOI:10.1146/annurev.arplant.57.032905.105159Clay K. Defensive symbiosis: A microbial perspective // Funct Ecol. – 2014. – № 28 (2). – Рр. 293–298. https://doi.org/10.1111/1365–2435.12258.Clay K. Defensive symbiosis: A microbial perspective. Funct Ecol. 2014: 28; 2: 293–298. https://doi.org/10.1111/1365–2435.12258Arnold A.E., Mejía L.C., Kyllo D., Rojas E.I., Maynard Z., Robbins N., Herre E.A. Fungal endophytes limit pathogen damage in a tropical tree // Proc Natl Acad Sci USA. – 2003. – № 100. – Рр. 15649–15654. https://doi.org/10.1073/pnas.2533483100.Arnold A.E., Mejía L.C., Kyllo D., Rojas E.I., Maynard Z., Robbins N., Herre E.A. Fungal endophytes limit pathogen damage in a tropical tree. Proc Natl Acad Sci USA. 2003; 100: 15649–15654. https://doi.org/10.1073/pnas.2533483100Lata R., Chowdhury S., Gond S.K., White J.F. Induction of abiotic stress tolerance in plants by endophytic microbes // Lett Appl Microbiol. – 2018. – № 66. – Рр. 268–276. https://doi.org/10.1111/lam.12855.Lata R., Chowdhury S., Gond S.K, White J.F. Induction of abiotic stress tolerance in plants by endophytic microbes. Lett Appl Microbiol. 2018; 66: 268–276. https://doi.org/10.1111/lam.12855Schulz B. and Boyle C. The endophytic continuum // Mycol Research. – 2005. – 5109. – 6. – 661–686. https://doi.org/10.1017/S095375620500273X.Schulz B. and Boyle C. The endophytic continuum. Mycol Research. 2005; 5109; 6: 661–686. https://doi.org/10.1017/S095375620500273XSchulz B., Guske S., Dammann U., Boyle C. Endophytehost interactions. II. Defining symbiosis ofthe endophyte host interaction // Symbiosis. – 1998. – № 25. – Рр. 213–227.Schulz B., Guske S., Dammann U., Boyle C. Endophytehost interactions. II. Defining symbiosis of the endophyte host interaction. Symbiosis. 1998; 25: 213–227.Woudenberg J.H.C., Sandoval-Denis M., Houbraken J., Seifert K.A., Samson R.A. Cephalotrichum and related synnematousfungi with notes on speciesfrom the built environment // Stud in Mycol. – 2017. – № 88. – Рр. 137–159. https://doi.org/10.1016/j.simyco.2017.09.001.Woudenberg J.H.C., Sandoval-Denis M., Houbraken J., Seifert K.A., Samson R.A. Cephalotrichum and related synnematous fungi with notes on species from the built environment. Stud in Mycol. 2017; 88: 137–159. https://doi.org/10.1016/j.simyco.2017.09.001Domsch K.H., Gams W., Anderson T.H. Compendium of soil fungi, 2nd Ed. IHW Verlag, Eching, Germany, 2007.Domsch K.H., Gams W., Anderson T.H. Compendium of soil fungi, 2nd Ed. IHW Verlag, Eching, Germany, 2007.Ghosta Y., Azizi R., Poursafar A. New species of synnematous fungi for Iran mycobiota // J of Plant Research 2020. – № 33. – Рр. 998–1009.Ghosta Y., Azizi R., Poursafar A. New species of synnematous fungi for Iran mycobiota. J of Plant Research. 2020; 33: 998–1009.Paripour Z., Davari M. and Asgari B. A new record of Cephalotichum olighotriphicum for mycobiota of Iran and Robinia pseudoacacia as a new host for this fungus. Proceedings of 4th Iranian Mycological Congress, Sari Agricultural Sciences and Natural Resources University. – Iran, 2019Paripour Z., Davari M. and Asgari B. A new record of Cephalotichum olighotriphicum for mycobiota of Iran and Robinia pseudoacacia as a new host for this fungus. Proceedings of 4th Iranian Mycological Congress, Sari Agricultural Sciences and Natural Resources University, Iran, 2019.Webster J., Weber R. Introduction to Fungi (3rd ed.) Cambridge University Press, Cambridge, UK, 2007.Webster J., Weber R. Introduction to Fungi (3rd ed.). Cambridge University Press, Cambridge, UK, 2007.Kusaba M. and Tsuge T. Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA // Curr Genet. – 1995. – № 28. – Рр. 491–498.Kusaba M. and Tsuge T. Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr Genet. 1995; 28: 491–498.Rzhetsky A., Nei M. ASimple Method for Estimating and Testing Minimum-Evolution Trees // Mol. Biol. Evol. – 1992. – № 9. – Р. 945.Rzhetsky A., Nei M. A Simple Method for Estimating and Testing Minimum-Evolution Trees. Mol. Biol. Evol. 1992; 9: 945.Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap //Evolution. – 1985. – № 39. –Рр. 783–791. https://doi.org/10.1111/j.1558–5646.1985.tb00420.x.Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985; 39: 783–791. https://doi.org/10.1111/j.1558–5646.1985.tb00420.xTamura K., Nei M., Kumar S. Prospects for Inferring Very Large Phylogenies by Using the Neighbor-Joining Method // Proc. Natl. Acad. Sci. – USA. – 2004. – № 101. – Рр. 11030–11035. https://doi.org/10.1073/pnas.0404206101.Tamura K., Nei M., Kumar S. Prospects for Inferring Very Large Phylogenies by Using the Neighbor-Joining Method. Proc. Natl. Acad. Sci. USA. 2004; 101: 11030–11035. https://doi.org/10.1073/pnas.0404206101Nei M. and Kumar S. Molecular Evolution and Phylogenetics; Oxford University Press: Oxford, UK.Nei M. and Kumar S. Molecular Evolution and Phylogenetics; Oxford University Press: Oxford, UK. ISBN0–19–513584–9, 2000.Saitou N. and Nei M. The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees // Mol. Biol. Evol. – 1987. – № 4. – С. 406–425.Saitou N. and Nei M. The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Mol. Biol. Evol. 1987; 4: 406–425.Letunic I. and Bork P. Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. NucleicAcids Res. – 2021. – № 2 (49). – Рр. 293–296. https://doi.org/10.1093/nar/gkab301.Letunic I. and Bork P. Interactive Tree of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Res. 2021; 2; 49: 293–296. https://doi.org/10.1093/nar/gkab301Zhang M., Sun X., Cui L., Yin Y., Zhao X., Pan S., Wang W. The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea // J Vis Exp. – 2018. – № 138. – 57675. https://dx.doi.org/10.3791/57675.Zhang M., Sun X., Cui L., Yin Y., Zhao X., Pan S., Wang W. The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea. J Vis Exp. 2018; 138; 57675. https://dx.doi.org/10.3791/57675Alijani N., Mamaghani A., Javan-Nikkhah M., De Respinis S., Pianta E. Endophytic Cephalotrichum spp. from Solanum tuberosum (potato) in Iran – a polyphasic analysis // Sydowia. – 2022. – № 74. – С. 287–302. http://dx.doi.org/10.12905/0380.sydowia74–2022–0287.Alijani N., Mamaghani A., Javan-Nikkhah M., De Respinis S., Pianta E. Endophytic Cephalotrichum spp. from Solanum tuberosum (potato) in Iran – a polyphasic analysis. Sydowia. 2022; 74: 287–302. http://dx.doi.org/10.12905/0380.sydowia74–2022–0287Lu H., Wei T., Lou H., Shu X., Chen Q. A Critical Review on Communication Mechanism within Plant-Endophytic Fungi Interactions to Cope with Biotic and Abiotic Stresses // J Fungi. – 2021. – № 7. – С. 719. https://doi.org/10.3390/jof7090719.Lu H., Wei T., Lou H., Shu X., Chen Q. A Critical Review on Communication Mechanism within Plant-Endophytic Fungi Interactions to Cope with Biotic and Abiotic Stresses. J Fungi. 2021; 7; 719. https://doi.org/10.3390/jof7090719Pfordt A., Schiwek S., Karlovsky P. and von Tiedemann A. Trichoderma Afroharzianum Ear Rot-A New Disease on Maize in Europe // Front. Agron. – 2020. – № 2. – 547758. https://doi.org/10.3389/fagro.2020.547758.Pfordt A., Schiwek S., Karlovsky P. and von Tiedemann A. Trichoderma Afroharzianum Ear Rot–A New Disease on Maize in Europe. Front. Agron. 2020; 2; 547758. https://doi.org/10.3389/fagro.2020.547758Thomma B.P.H.J. Alternaria Spp.: From General Saprophyte to Specific Parasite // Mol Pl Path. – 2003. – № 4 (4). – С. 225–236. https://doi.org/10.1046/j.1364–3703.2003.00173.xThomma B.P.H.J. Alternaria Spp.: From General Saprophyte to Specific Parasite. Mol Pl Path. 2003; 4; 4: 225–236 https://doi.org/10.1046/j.1364–3703.2003.00173.xMeena M., Gupta S.K., Swapnil P., Zehra A., Dubey M.K., Upadhyay R.S. Alternaria Toxins: Potential Virulence Factors and Genes Related to Pathogenesis // Front in Microbiol. – 2017. – № 8. https://doi.org/10.3389/fmicb.2017.01451.Meena M., Gupta S.K., Swapnil P., Zehra A., Dubey M.K., Upadhyay R.S. Alternaria Toxins: Potential Virulence Factors and Genes Related to Pathogenesis. Front in Microbiol. 2017; 8. https://doi.org/10.3389/fmicb.2017.01451Brasier C.M. Rapid Evolution of Introduced Plant Pathogens via Interspecific Hybridization: Hybridization Is Leading to Rapid Evolution of Dutch Elm Disease and Other Fungal Plant Pathogens // BioScience. – 2001. – № 51. – Рр. 123–133. https://doi.org/10.1641/0006–3568(2001)051[0123:REOIPP]2.0.CO;2.Brasier C.M. Rapid Evolution of Introduced Plant Pathogens via Interspecific Hybridization: Hybridization Is Leading to Rapid Evolution of Dutch Elm Disease and Other Fungal Plant Pathogens. BioScience. 2001; 51: 123–133. https://doi.org/10.1641/0006–3568(2001)051[0123:REOIPP]2.0.CO;2Brasier C.M. Episodic selection as a force in fungal microevolution, with special reference to clonal speciation and hybrid introgression // Canad J of Botany. – 73. – Рр. 1213–1221. https://doi.org/10.1139/b95–381.Brasier C.M. Episodic selection as a force in fungal microevolution, with special reference to clonal speciation and hybrid introgression. Canad J of Botany. 1995; 73: 1213–1221. https://doi.org/10.1139/b95–381

The authors declare that there are no conflicts of interest present.