izvestiiatimacadИзвестия Тимирязевской сельскохозяйственной академииIZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY0021-342XФГБОУ ВО РГАУ-МСХА имени К.А. Тимирязева10.26897/0021-342X-2022-4-82-93izvestiiatimacad-319Research ArticleЗЕМЛЕДЕЛИЕ, РАСТЕНИЕВОДСТВО, ЗАЩИТА РАСТЕНИЙAGRONOMY, CROP PRODUCTION, PLANT PROTECTIONОценка бактерицидного действия фунгицида Ридомил Голд Р против возбудителей черной ножки картофеляEffi cacy of ridomil gold r as bactericide against potato black leg pathogenДацюкА. А.DatsyukA. A.

Дацюк Анна Андреевна, аспирант кафедры защиты растений

127550, г. Москва, ул. Тимирязевская, 49

Anna A. Datsyuk, PhD student, Department of Plant protection

127550, Moscow, Timiryzevskaya Str., 49

annadacyk@rgau-msha.ruДжалиловФ. С.-У.DzhalilovF. S.-U.

Джалилов Февзи Сеид-Умерович, д-р биол. наук, заведующий кафедрой защиты растений

127550, г. Москва, ул. Тимирязевская, 49

Fevzi S.-U. Dzhalilov, DSc (Bio), Head of the Department of PlantProtection

127550, Moscow, Timiryzevskaya Str., 49

dzhalilov@rgau-msha.ruРоссийский государственный аграрный университет – МСХА имени К.А. ТимирязеваРоссияRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian Federation202226032023048293Copyright © Дацюк А.А., Джалилов Ф.С., 20232023Дацюк А.А., Джалилов Ф.С.Datsyuk A.A., Dzhalilov F.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiia.timacad.ru/jour/article/view/319

С целью разработки мер защиты картофеля от черной ножки проведена оценка бактерицидного действия фунгицида Ридомил Голд Р, ВДГ. Тестирование проводили на трех изолятах фитопатогенных бактерий возбудителей черной ножки картофеля: Dickeya chrysanthemi (DSM 4610, Германия), Pectobacterium carotovorum subsp. brasiliensis (РФ, Омская область), Pectobacterium wasabiae (РФ, Кемеровская область). Оценку проводили in vitro путем подсчета колоний бактерий, посеянных на среду КГА с добавлением фунгицида в концентрациях 0,1; 0,2; 0,4; 0,6 и 1% по препарату. Установлено, что фунгицид Ридомил Голд Р показывает 100%-ную биологическую эффективность по отношению ко всем исследуемым штаммам бактерий уже при концентрации препарата в среде 0,2% (LD100 = 0,2%). Дополнительно была проведена серия опытов с применением метода отпечатков листьев картофеля, обработанных бактериальной суспензией патогенов, на питательную среду с добавлением 1%-ного рабочего раствора фунгицида. Показано, что кратковременный контакт препарата с эпифитной популяцией листа картофеля приводит к полной гибели бактерий.

Bactericidal activity of copper-containing fungicide Ridomil Gold R (WDG) was tested in framework of potato black leg control. Three strains of bacteria causing potato black leg were employed in the trials: Dickeya chrysanthemi (DSM 4610, Germany), Pectobacterium carotovorum subsp. brasiliensis (Russia, Omsk region), Pectobacterium wasabiae (Russia, Kemerovo reg.). The activity was estimated in vitro by counting colony number on PDA agar with 0.1, 0.2, 0.4, 0.6, and 1.0% of the fungicide. According to the results, for all the tested bacteria Ridomil Gold R had LD100 = 0.2%. Additional test has been done by potato leafprint method, after spraying leaves by bacterial suspension. The leaves were imprinted on PDA agar with 1% fungicide. No viable bacteria were found after incubation.

болезни растенийчерная ножка картофеляфунгицидыРидомил Голд РDickeya chrysanthemiPectobacterium carotovorum subsp. brasiliensisPectobacterium wasabiaeblackleg of potatosoft-rot bacterial pathogensplant protectionfungicidescopper preparationsRidomil Gold RDickeya chrysanthemiPectobacterium carotovorum subsp. brasiliensisPectobacterium wasabiaeReferencesABD EL-RAHMAN, A.F. Evaluation of some fungicides effectiveness in control of blackleg and common scab of potato / A.F. ABD EL-RAHMAN, A.A. EL-KAFRAWY, O.A. ABD EL-HAFEZ, A.B.D. EL-GHANY // Egyptian Journal of Agricultural Research. – 2018. – Т. 96, № 4. – С. 1307–1323.ABD EL-RAHMAN A. F., EL-KAFRAWY, A. A., ABD EL-HAFEZ, O. A., EL-GHANY, A. B.D. Evaluation of some fungicides effectiveness in control of blackleg and common scab of potato // Egyptian Journal of Agricultural Research. – 2018. – Т. 96. – № 4. – С. 1307–1323.Alfano J.R. Type III secretion system effector proteins: double agents in bacterial disease and plant defense / J.R. Alfano, A. Collmer // Annu. Rev. Phytopathol. – 2004. – Т. 42. – С. 385–414.Alfano J.R., Collmer A. Type III secretion system effector proteins: double agents in bacterial disease and plant defense // Annu. Rev. Phytopathol. – 2004. – T. 42. – S. 385–414.Burgess P.J. Contamination and subsequent multiplication of soft rot erwinias on healthy potato leaves and debris after haulm destruction / P.J. Burgess, J.P. Blakeman M.C.M. Perombelon // Plant Pathology. – 1994. – Т. 43, № 2. – С. 286–299.Burgess P.J., Blakeman J.P., Perombelon, M.C.M. Contamination and subsequent multiplication of soft rot erwinias on healthy potato leaves and debris after haulm destruction // Plant Pathology. – 1994. – T. 43. – № . 2. – S. 286–299.Charkowski A. The role of secretion systems and small molecules in soft-rot Enterobacteriaceae pathogenicity / A. Charkowski, C. Blanco, G. Condemine, D. Expert, T. Franza, C. Hayes, I. Yedidia // Annual review of phytop athology. – 2012. – Т. 50. – С. 425–449.Charkowski A., Blanco C., Condemine G., Expert D., Franza T., Hayes C., Yedidia I. The role of secretion systems and small molecules in soft-rot Enterobacteriaceae pathogenicity // Annual review of phytopathology. – 2012. – Т. 50. – С. 425–449.Czajkowski R. Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review / R. Czajkowski, M.C.M. Pérombelon S. Jafra, E. Lojkowska, M. Potrykus, Wolf J.M. Van Der W. Sledz // Annals of Applied Biology. – 2015. – Т. 166, № 1. – С. 18–38.Czajkowski R., Pérombelon M.C. M., Jafra S., Lojkowska E., Potrykus M., Van der Wolf J.M., Sledz W. Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review // Annals of Applied Biology. – 2015. – Т. 166. – № . 1. – С. 18–38.Czajkowski R. Systemic colonization of potato plants by a soilborne, green fluorescent protein-tagged strain of Dickeya sp. biovar 3 / R. Czajkowski, W.J. De Boer H. Velvis, J.M. Van der Wolf // Phytopathology. – 2010. – Т. 100, № 2. – С. 134–142.Czajkowski R., De Boer W.J., Velvis H., Van der Wolf J.M. Systemic colonization of potato plants by a soilborne, green fluorescent protein-tagged strain of Dickeya sp. biovar 3 // Phytopathology. – 2010. – Т. 100. – № . 2. – С. 134–142.Euphresco, 2013. Dickeya species in potato and management strategies (Dickeyaspp). Final report. – URL: https://www.euphresco.net/projects/portfolio.Euphresco, 2013. Dickeya species in potato and management strategies (Dickeya spp). Final report, https: https://www.euphresco.net/projects/portfolioEuphresco-II, 2015. Assessment of Dickeya sp. and Pectobacterium sp. on potatoes and ornamentals (Dickeya). Final report. – URL: https://www.euphresco.net/projects/portfolio.Euphresco-II, 2015. Assessment of Dickeya sp. and Pectobacterium sp. on potatoes and ornamentals (Dickeya). Final report, https: https://www.euphresco.net/projects/portfolioGraham D.C. Potential spread of Erwinia spp. in aerosols / D.C. Graham, M.D. Harrison // Phytopathology. – 1975. – Т. 65, № 6. – С. 739–741.Graham D.C., Harrison M.D. Potential spread of Erwinia spp. in aerosols // Phytopathology. – 1975. – Т. 65. – № . 6. – С. 739–741.Greiner B.W. Inoculation and spread of Dickeya in potatoes. – North Dakota State University, 2019.Greiner B.W. Inoculation and spread of Dickeya in potatoes: dis. – North Dakota State University, 2019.Hu, J.H. Mefenoxam sensitivity and fitness analysis of Phytophthora nicotianae isolates from nurseries in Virginia, USA / J.H. Hu, C.X. Hong, E.L. Stromberg, G.W. Moorman // Plant Pathology. – 2008. – Т. 57, № 4. – С. 728–736.Hu J.H., Hong C.X., Stromberg E.L., Moorman G.W. Mefenoxam sensitivity and fitness analysis of Phytophthora nicotianae isolates from nurseries in Virginia, USA // Plant Pathology. – 2008. – Т. 57. – № . 4. – С. 728–736.Hélias V. Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya / V. Hélias, P. Hamon, E. Huchet, J.M. Van der Wolf D. Andrivon // Plant pathology. – 2012. – Т. 61, № 2. – С. 339–345.Hélias V., Hamon P., Huchet E., Van der Wolf J.M., Andrivon D. Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya // Plant pathology. – 2012. – Т. 61. – № . 2. – С. 339–345.Jatav M.K. Impact of Climate Change on Potato Production in India / M.K. Jatav, V.K. Dua, P.M. Govindakrishnan, R.P. Sharma // Sustainable Potato Production and the Impact of Climate Change. – IGI Global, 2017. – С. 87–104.Jatav M.K., Dua V.K., Govindakrishnan P.M., Sharma R.P. Impact of Climate Change on Potato Production in India // Sustainable Potato Production and the Impact of Climate Change. – IGI Global, 2017. – С. 87–104.Karjalainen R. Piilevän tyvimädän tunnistaminen perunasta PCR-tekniikalla ja taudin torjuntamahdollisuudet / R. Karjalainen, S. Lehtimäki, M. Toivonen // Kohti huippulaatuista siemenperunaa. – 2000. – С. 47.Karjalainen R., Lehtimäki S., Toivonen M. Piilevän tyvimädän tunnistaminen perunasta PCR-tekniikalla ja taudin torjuntamahdollisuudet // Kohti huippulaatuista siemenperunaa. – 2000. – S. 47.Kastelein P. Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri / P. Kastelein, M.G. Förch, M.C. Krijger, P.S. Van der Zouwen W. Van den Berg J.M. Van der Wolf // Canadian Journal of Plant Pathology. – 2021. – T. 43, № 1. – S. 1–15.Kastelein P., Förch M.G., Krijger M.C., Van der Zouwen P.S., Van den Berg W., Van der Wolf J.M. Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri // Canadian Journal of Plant Pathology. – 2021. – T. 43. – № . 1. – S. 1–15.Khayi S. Complete genome anatomy of the emerging potato pathogen Dickeya solani type strain IPO 2222 T / S. Khayi, P. Blin, T.M. Chong, K.G. Chan, D. Faure // Standards in genomic sciences. – 2016. – Т. 11, № 1. – С. 1–6.Khayi S., Blin P., Chong T.M., Chan K.G., Faure D. Complete genome anatomy of the emerging potato pathogen Dickeya solani type strain IPO 2222 T // Standards in genomic sciences. – 2016. – Т. 11. – № . 1. – С. 1–6.Krzyzanowska D.M. Compatible mixture of bacterial antagonists developed to protect potato tubers from Soft Rot caused by Pectobacterium spp. and Dickeya spp / D.M. Krzyzanowska, T. Maciag, J. Siwinska, M. Krychowiak, S. Jafra, R. Czajkowski // Plant disease. – 2019. – T. 103, № 6. – S. 1374–1382.Krzyzanowska D.M., Maciag T., Siwinska J., Krychowiak M., Jafra S. Czajkowski, R. Compatible mixture of bacterial antagonists developed to protect potato tubers from Soft Rot caused by Pectobacterium spp. and Dickeya spp // Plant disease. – 2019. – T. 103. – № . 6. – S. 1374–1382.Ma, B. Host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya / B. Ma, M.E. Hibbing, H.S. Kim, R.M. Reedy, I. Yedidia, J. Breuer, A.O. Charkowski // Phytopathology. – 2007. – Т. 97, № 9. – С. 1150–1163.Ma B., Hibbing M.E., Kim H.S., Reedy R.M., Yedidia I., Breuer J., Charkowski A.O. Host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya // Phytopathology. – 2007. – Т. 97. – № . 9. – С. 1150–1163.Mansfield J. Top 10 plant pathogenic bacteria in molecular plant pathology / J. Mansfield, S. Genin, S. Magori, V. Citovsky, M. Sriariyanum, P. Ronald, G.D. Foster // Molecular plant pathology. – 2012. – T. 13, № 6. – S. 614–629.Mansfield J., Genin S., Magori S., Citovsky V., Sriariyanum M., Ronald P., Foster G.D. Top 10 plant pathogenic bacteria in molecular plant pathology // Molecular plant pathology. – 2012. – T. 13. – № . 6. – S. 614–629.Papenfort K. Quorum sensing signal – response systems in Gram-negative bacteria / K. Papenfort, B.L. Bassler // Nature Reviews Microbiology. – 2016. – Т. 14, № . 9. – С. 576–588.Papenfort K., Bassler B.L. Quorum sensing signal–response systems in Gram-negative bacteria // Nature Reviews Microbiology. – 2016. – T. 14. – № . 9. – S. 576–588.Pasanen M. Characterization of Pectobacterium strains causing soft rot and blackleg of potato in Finland, 2020.Pasanen M. Characterization of Pectobacterium strains causing soft rot and blackleg of potato in Finland. – 2020.Pérombelon M.C.M. Potato blackleg: epidemiology, host-pathogen interaction and control // Netherlands Journal of Plant Pathology. – 1992. – Т. 98, № 2. – С. 135–146.Pérombelon M.C.M. Potato blackleg: epidemiology, host-pathogen interaction and control // Netherlands Journal of Plant Pathology. – 1992. – T. 98. – № . 2. – S. 135–146.Põllumaa L. Quorum sensing and expression of virulence in pectobacteria / L. Põllumaa, T. Alamäe, A. Mäe // Sensors. – 2012. – Т. 12, № 3. – С. 3327–3349.Põllumaa L., Alamäe T., Mäe A. Quorum sensing and expression of virulence in pectobacteria // Sensors. – 2012. – T. 12. – № . 3. – S. 3327–3349.Rusjan D. Copper in horticulture // Fungicides for plant and animal diseases. – IntechOpen, 2012.Rusjan D. Copper in horticulture // Fungicides for plant and animal diseases. – IntechOpen, 2012.Samson R. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. / R. Samson, J.B. Legendre, R. Christen, M. Fischer-Le Saux W. Achouak, L. Gardan // International Journal of Systematic and Evolutionary Microbiology. – 2005. – Т. 55, № 4. – С. 1415–1427.Samson R., Legendre J.B., Christen R., Fischer-Le Saux M., Achouak W., Gardan L. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. // International Journal of Systematic and Evolutionary Microbiology. – 2005. – Т. 55. – № . 4. – С. 1415–1427.Toth I.K. Dickeya species: an emerging problem for potato production in Europe / I.K. Toth, J.M. Van der Wolf G. Saddler, E. Lojkowska, V Hélias., M. Pirhonen, J.G. Elphinstone // Plant Pathology. – 2011. – T. 60, № 3. – S. 385–399.Toth I.K., Van der Wolf J.M., Saddler G., Lojkowska E., Hélias V., Pirhonen M., Elphinstone J.G. Dickeya species: an emerging problem for potato production in Europe // Plant Pathology. – 2011. – T. 60. – № . 3. – S. 385–399.Виноградова С.В. Полногеномное секвенирование фитопатогенных бакте- рий / С.В. Виноградова, Е.И. Кырова, А.Н. Игнатов // Защита картофеля. – 2014. – № . 2. – С. 15–17.Vinogradova S.V., Kyrova E.I., Ignatov A.N. Polnogenomnoe sekvenirovanie fitopatogennyh bakterij // Zashchita kartofelya. – 2014. – № . 2. – S. 15–17.Ерохова М.Д. «Черная ножка» – опасное для отечественного картофелеводства заболевание / М.Д. Ерохова, М.А. Кузнецова // Аграрная наука. – 2019. – Т. 3. – С. 44–48.Erohova M.D., Kuznecova M.A. “CHyornaya nozhka”-opasnoe dlya otechestvennogo kartofelevodstva zabolevanie // Agrarnaya nauka. – 2019. – T. 3. – S. 44–48.Игнатов А.Н. Распространение бактериальных и фитоплазменных болезней растений в России / А.Н. Игнатов, М.С. Егорова, М.В. Ходыкина // Защита и карантин растений. – 2015. – № 5. – С. 6–10.Ignatov A.N., Egorova M.S., Hodykina M.V. Rasprostranenie bakterial’nyh i fitoplazmennyh boleznej rastenij v Rossii // Zashchita i karantin rastenij. – 2015. – № . 5. – S. 6–10.Игнатов А.Н. Динамика видового состава патогенов картофеля в Европейской части РФ / А.Н. Игнатов, Ю.С. Панычева, М.В. Воронина, Д.М. Васильев Ф.С.У. Джалилов // Картофель и овощи. – 2019. – Т. 9. – С. 28–32.Ignatov A.N., Panycheva, YU. S., Voronina M.V., Vasil’ev D. M., Dzhalilov F.S.U. Dinamika vidovogo sostava patogenov kartofelya v evropejskoj chasti RF // Kartofel’ i ovoshchi. – 2019. – T. 9. – S. 28–32.Карлов А.Н. Dickeya dianthicola-новый для России бактериальный патоген картофеля / А.Н. Карлов, В.С. Зотов, Э.Ш. Пехтерева, Е.В. Матвеева Ф.С.У. Джалилов И.А. Фесенко, Г.И. Карлов // Известия ТСХА. – 2010. – № 3. – С. 134–141.Karlov A.N., Zotov V.S., Pekhtereva E.Sh., Matveeva E.V., Dzhalilov F.S.U., Fesenko I.A., Karlov G.I. Dickeya dianthicola-novyj dlya Rossii bakterial’nyj patogen kartofelya // Izvestiya Timiryazevskoj sel’skohozyajstvennoj akademii. – 2010. – № . 3. – S. 134–141.Ковтунов Е.А. Ферменты деградации рамногалактуронана как факторы вирулентности фитопатогенной бактерии Pectobacterium atrosepticum / Е.А. Ковтунов, В.Ю. Горшков, Н.Е. Гоголева, О.Е. Петрова, Е.В. Осипова, Ч.Б. Нуриахметова, Ю.В. Гоголев // Сельскохозяйственная биология. – 2019. – Т. 54, № 3. – С. 566–574.Kovtunov E.A., Gorshkov V.Yu., Gogoleva N.E., Petrova O.E., Osipova E.V., Nuriahmetova, Ch. B., Gogolev, Yu.V. Fermenty degradacii ramnogalakturonana kak faktory virulentnosti fitopatogennoj bakterii Pectobacterium atrosepticum // Sel’skohozyajstvennaya biologiya. – 2019. – T. 54. – № . 3. – S. 566–574.Мыца Е.Д. Новый препарат «Зерокс» – оценка фунгицидного и бактерицидного эффекта in vitro / Е.Д. Мыца, С.Н. Еланский, Л.Ю. Кокаева, М.А. Побединская, А.Н. Игнатов, М.А. Кузнецова, Ю.А. Крутяков // Достижения науки и техники АПК. – 2014. – № 12. – С. 16–19.Myca E.D., Elanskij S.N., Kokaeva L.Yu., Pobedinskaya M.A., Ignatov A.N., Kuznecova M.A., Krutyakov, Yu.A. Novyj preparat «zeroks»-ocenka fungicidnogo i baktericidnogo effekta in vitro // Dostizheniya nauki i tekhniki APK. – 2014. – № . 12. – S. 16–19.

The authors declare that there are no conflicts of interest present.