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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">izvestiiatimacad</journal-id><journal-title-group><journal-title xml:lang="ru">Известия Тимирязевской сельскохозяйственной академии</journal-title><trans-title-group xml:lang="en"><trans-title>IZVESTIYA OF TIMIRYAZEV AGRICULTURAL ACADEMY</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0021-342X</issn><publisher><publisher-name>ФГБОУ ВО РГАУ-МСХА имени К.А. Тимирязева</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.26897/0021-342X-2025-6-79-91</article-id><article-id custom-type="elpub" pub-id-type="custom">izvestiiatimacad-1058</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗЕМЛЕДЕЛИЕ, РАСТЕНИЕВОДСТВО, ЗАЩИТА РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>AGRONOMY, CROP PRODUCTION, PLANT PROTECTION</subject></subj-group></article-categories><title-group><article-title>Эффективность применения биологических фунгицидов против корневой гнили ячменя озимого на юге России</article-title><trans-title-group xml:lang="en"><trans-title>Efficacy of biological fungicides against winter barley root rot in the south of Russia</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Волкова</surname><given-names>Г. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Volkova</surname><given-names>G. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Галина Владимировна Волкова, д-р биол. наук, член-корр. РАН, главныйнаучный сотрудник, руководитель лаборатории</p><p>лаборатория иммунитета растений к болезням</p><p>350039; ул. имени Калинина, 62; Краснодар</p></bio><bio xml:lang="en"><p>Galina V. Volkova, DSc (Bio), Corresponding Member of the Russian Academy of Sciences, Head of the Laboratory</p><p>Laboratory of Plant Immunity to Diseases</p><p>350039; 62 Kalinina St.; Krasnodar</p></bio><email xlink:type="simple">galvol.bpp@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Яхник</surname><given-names>Я. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Yakhnik</surname><given-names>Ya. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Яна Викторовна Яхник, научный сотрудник</p><p>лаборатория иммунитета растений к болезням</p><p>350039; ул. имени Калинина, 62; Краснодар</p></bio><bio xml:lang="en"><p>Yana V. Yakhnik, Research Associate</p><p>Laboratory of Plant Immunity to Diseases</p><p>350039; 62 Kalinina St.; Krasnodar</p></bio><email xlink:type="simple">yahnik1@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кустадинчев</surname><given-names>А. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Kustadinchev</surname><given-names>A. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Александр Дмитриевич Кустадинчев, младший научный сотрудник</p><p>лаборатория иммунитета растений к болезням</p><p>350039; ул. имени Калинина, 62; Краснодар</p></bio><bio xml:lang="en"><p>Aleksandr D. Kustadinchev, Junior Research Associate</p><p>Laboratory of Plant Immunity to Diseases</p><p>350039; 62 Kalinina St.; Krasnodar</p></bio><email xlink:type="simple">skustadinchev@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный научный центр биологической защиты растений</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Scientific Center for Biological Plant Protection</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>25</day><month>01</month><year>2026</year></pub-date><volume>0</volume><issue>6</issue><fpage>79</fpage><lpage>91</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Волкова Г.В., Яхник Я.В., Кустадинчев А.Д., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Волкова Г.В., Яхник Я.В., Кустадинчев А.Д.</copyright-holder><copyright-holder xml:lang="en">Volkova G.V., Yakhnik Y.V., Kustadinchev A.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://izvestiia.timacad.ru/jour/article/view/1058">https://izvestiia.timacad.ru/jour/article/view/1058</self-uri><abstract><p>   Цель исследований – изучить эффективность биологических фунгицидов против возбудителей корневых гнилей озимого ячменя для дальнейшего их практического применения в экологизированных технологиях защиты Краснодарского края.</p><p>   Опыты выполняли на базе Федерального государственного бюджетного научного учреждения «Федеральный научный центр биологической защиты растений» в условиях полевого инфекционного питомника в 2023–2025 гг.</p><p>   Объект исследований – возбудители корневых гнилей ячменя озимого фузариозной и гельминтоспориозной этиологии.</p><p>   Для исследований отобраны препараты, содержащие различные агенты биологического происхождения, и их метаболиты (Геостим Фит, А, Ж 2 л/т; Алирин Б, Ж, 2 л/га; Псевдобактерин-2, Ж, 1 л/га; Стернифаг, СП, 80 г/т; Трихоцин, СП, 20 г/т; опытный образец ФГБНУ ФНЦБЗР на основе Bacillus velezenzis BZR336 g, Ж, 3 л/т; опытный образец ФГБНУ ФНЦБЗР на основе Pseudomonas chlororaphis BZR245-F, Ж, 3 л/т; Оргамика Ф, Ж, 0,7 л/т; Скарлет, МЭ, 0,4 л/т (химический эталон)). Максимальная эффективность против заражения семян фузариозной инфекцией выявлена после обработки препаратами Трихоцин, СП (94,5%), Геостим Фит, А, Ж (92,4 %), Алирин-Б, Ж (92,3 %) и Оргамика Ф, Ж (91,1 %). Лабораторная и полевая всхожесть определена как максимальная после обработки семян препаратами на основе: Trichoderma asperellum – 96 % (Оргамика Ф, Ж); Bacillus velezenzis – 95 % (опытный образец ФГБНУ ФНЦБЗР на основе Bacillus velezenzis BZR336 g, Ж); Pseudomonas chlororaphis – 95 % (опытный образец ФГБНУ ФНЦБЗР на основе Pseudomonas chlororaphis BZR245-F, Ж). Наибольшие показатели густоты стояния выявлены на опытных делянках с препаратами Алирин-Б, Ж, Геостим Фит, А, Ж (по 488 раст/м2), Стернифаг, СП (по 485 раст/м2), Псевдобактерин-2, Ж и эталоном Скарлет, МЭ (по 484 раст/м2). Определены высокие показатели эффективности против корневых и прикорневых гнилей препаратов на основе Bacillus subtilis Алирин-Б, Ж (68,3 %), на основе Pseudomonas chlororaphis BZR245-F, Ж (65,2 %) и Trichoderma harzianum Стернифаг, СП (60,3 %). Исследования эффективности биологических препаратов для защиты ячменя от корневых гнилей крайне важны для практического применения в агропромышленном комплексе региона и страны.</p></abstract><trans-abstract xml:lang="en"><p>   The aim of this research was to evaluate the efficacy of biological fungicides against winter barley root rot pathogens for their subsequent practical application in ecological protection technologies within the Krasnodar Territory.</p><p>   The experiments were conducted at the Federal Scientific Center for Biological Plant Protection (FRCBPP) under field infection nursery conditions during 2023–2025.</p><p>   The object of study comprised winter barley root rot pathogens of Fusarium and Helminthosporium etiology.</p><p>   Selected for the study were preparations containing various agents of biological origin and their metabolites: Geostim Fit, A, L (2 l/t); Alirin B, L (2 l/ha); Pseudobacterin-2, L (1 l/ha); Sterniphag, WP (80 g/t); Trichocin, WP (20 g/t); an experimental sample from the FR-CBPP based on Bacillus velezensis BZR336 g, L (3 l/t); an experimental sample from the FRCB-PP based on Pseudomonas chlororaphis BZR245-F, L (3 l/t); Orgamica F, L (0.7 l/t); and Scarlet, ME (0.4 l/t) (chemical standard). The maximum efficacy against Fusarium seed infection was observed after treatment with Trichocin, WP (94.5 %), Geostim Fit, A, L (92.4 %), Alirin-B, L (92.3 %), and Organica F, L (91.1 %). Laboratory and field germination rates were highest after seed treatment with preparations based on Trichoderma asperellum – 96 % (Organica F, L), Bacillus velezensis – 95 % (experimental sample from the FRCBPP based on Bacillus velezensis BZR336 g, L), and Pseudomonas chlororaphis – 95 % (experimental sample from the FRCBPP based on Pseudomonas chlororaphis BZR245-F, L). The highest plant stand densities were recorded on experimental plots treated with Alirin-B, L, Geostim Fit, A, L (488 plants/m2), Sterniphag, WP (485 plants/m2), Pseudobacterin-2, L, and the Scarlet, ME standard (484 plants/m2). High efficacy rates against root and radical rots were determined for preparations based on Bacillus subtilis Alirin-B, L (68.3 %), Pseudomonas chlororaphis BZR245-F, L (65.2 %), and Trichoderma harzianum Sterniphag, WP (60.3 %). Research into the efficacy of biological preparations for protecting barley from root rot is crucial for practical application within the agro-industrial sector of both the region and the country.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>озимый ячмень</kwd><kwd>биологические фунгициды</kwd><kwd>корневые гнили</kwd><kwd>экологизация земледелия</kwd></kwd-group><kwd-group xml:lang="en"><kwd>winter barley</kwd><kwd>biological fungicides</kwd><kwd>root rot</kwd><kwd>ecologization of agriculture</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследования выполнены при финансовой поддержке Кубанского научного фонда в рамках научного проекта № Н-24.1/43</funding-statement><funding-statement xml:lang="en">This research was funded by the Kuban Scientific Foundation within the framework of scientific project No. H-24.1/43</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Овсянкина А.В. Корневые гнили зерновых // Теория и практика борьбы с паразитарными болезнями. 2012. № 13. С. 300-303. EDN: ZNHSSN</mixed-citation><mixed-citation xml:lang="en">Ovsyankina A.V. Root rot of cereals. Teoriya i praktika borby s parazitarnymi boleznyami. 2012:(13):300-303. (In Russ.) EDN: ZNHSSN</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Zhukova L.V., Stankevych S.V., Turenko V.P., et al. Root Rots of Spring Barley, Their Harmfulness and the Basic Effective Protection Measures. Ukrainian Journal of Ecology. 2019;9(2): 232-238</mixed-citation><mixed-citation xml:lang="en">Zhukova L.V., Stankevych S.V., Turenko V.P. et al. Root rots of spring barley, their harmfulness and the basic effective protection measures. Ukrainian Journal of Ecology. 2019;(9):232-238.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Жуковский А.Г., Крупенько Н.А., Буга С.Ф. и др. Корневая гниль зерновых культур и роль инфицированности семян в ее развитии // Защита растений. 2022. № 42. С. 84-95. EDN: YYEFNN</mixed-citation><mixed-citation xml:lang="en">Zhukovsky A.G., Krupenko N.A., Buga S.F. et al. Root rot of grain crops and seed affection role in its severity. Zashchita rasteniy. 2022;(42):84-95. (In Russ.) EDN: YYEFNN</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Ганнибал Ф.Б., Полуэктова Е.В., Лукьянец Я.В. и др. Ассоциированные с ячменем микромицеты и их значимость как возбудителей болезней в России // Вестник зашиты растений. 2023. № 4. С. 172-186. doi: 10.31993/2308-6459-2023-106-4-16116</mixed-citation><mixed-citation xml:lang="en">Hannibal F.B., Poluektova E.V., Lukyanets Y.V. et al. Micromycetes associated with barley and their significance as pathogens in Russia. Plant Protection News. 2023;106(4):172-186. (In Russ.) doi: 10.31993/2308-6459-2023-106-4-16116</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Saad A., Macdonald B., Martin A. et al. Winter cereal reactions to common root rot and crown rot pathogens in the field. Agronomy. 2022;12(10):2571. doi: 10.3390/agronomy12102571</mixed-citation><mixed-citation xml:lang="en">Saad A., Macdonald B., Martin A. et al. Winter cereal reactions to common root rot and crown rot pathogens in the field. Agronomy. 2022;12(10):2571. doi: 10.3390/agronomy12102571</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Yin Y., Miao J., Shao W. et al. Fungicide resistance: Progress in understanding mechanism, monitoring and management. Phytopathology. 2023;113(4):707-718. doi: 10.1094/PHYTO-10-22-0370-KD</mixed-citation><mixed-citation xml:lang="en">Yin Y., Miao J., Shao W. et al. Fungicide resistance: Progress in understanding mechanism, monitoring and management. Phytopathology. 2023;113(4):707-718. doi: 10.1094/PHYTO-10-22-0370-KD</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Dutilloy E., Oni F.E., Esmaeel Q. et al. Plant beneficial bacteria as bioprotectants against wheat and barley diseases. Journal of Fungi. 2022;8(6):632. doi: 10.3390/jof8060632</mixed-citation><mixed-citation xml:lang="en">Dutilloy E., Oni F.E., Esmaeel Q. et al. Plant beneficial bacteria as bioprotectants against wheat and barley diseases. Journal of Fungi. 2022;8(6):632. doi: 10.3390/jof8060632</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Kiesewalter H.T., Lozano-Andrade C.N., Wibowo M. et al. Genomic and chemical diversity of Bacillus subtilis secondary metabolites against plant pathogenic fungi. Msystems. 2021;6(1): e00770-20. doi: 10.1128/msystems.00770-20</mixed-citation><mixed-citation xml:lang="en">Kiesewalter H.T., Lozano-Andrade C.N., Wibowo M. et al. Genomic and chemical diversity of Bacillus subtilis secondary metabolites against plant pathogenic fungi. Msystems. 2021;6(1): e00770-20. doi: 10.1128/msystems.00770-20</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Romero D., Traxler M.F., Lopez D. et al. Antibiotics as signal molecules. Chemical Reviews. 2011;111(9):5492-5505. doi: 10.1021/cr2000509</mixed-citation><mixed-citation xml:lang="en">Romero D., Traxler M.F., Lopez D. et al. Antibiotics as signal molecules. Chemical Reviews. 2011;111(9):5492-5505. doi: 10.1021/cr2000509</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Торопова Е.Ю., Селюк М.П., Казакова О.А. и др. Факторы индукции супрессивности почвы агроценозов // Агрохимия. 2017. № 4. С. 51-64. EDN: YRCQIR</mixed-citation><mixed-citation xml:lang="en">Toropova E.Y., Selyuk M.P., Kazakova O.A. et al. Suppressive induction factors in agrocenosis soil. Agrohimia. 2017;(4):51-64. (In Russ.) EDN: YRCQIR</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Asad S.A. Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases - A review. Ecological Complexity. 2022;49:100978. doi: 10.1016/j.ecocom.2021.100978</mixed-citation><mixed-citation xml:lang="en">Asad S.A. Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases - A review. Ecological Complexity. 2022;49:100978. doi: 10.1016/j.ecocom.2021.100978</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Yue Y., Wang Z., Zhong T. et al. Antifungal mechanisms of volatile organic compounds produced by Pseudomonas fluorescens ZX as biological fumigants against Botrytis cinerea. Microbiological Research. 2023;267:127253. doi: 10.1016/j.micres.2022.127253</mixed-citation><mixed-citation xml:lang="en">Yue Y., Wang Z., Zhong T. et al. Antifungal mechanisms of volatile organic compounds produced by Pseudomonas fluorescens ZX as biological fumigants against Botrytis cinerea. Microbiological Research. 2023;267:127253. doi: 10.1016/j.micres.2022.127253</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Wei D., Zhu D., Zhang Y. et al. Characterization of rhizosphere Pseudomonas chlororaphis IRHB3 in the reduction of Fusarium root rot and promotion of soybean growth. Biological Control. 2023;186:105349. doi: 10.1016/j.biocontrol.2023.105349</mixed-citation><mixed-citation xml:lang="en">Wei D., Zhu D., Zhang Y. et al. Characterization of rhizosphere Pseudomonas chlororaphis IRHB3 in the reduction of Fusarium root rot and promotion of soybean growth. Biological Control. 2023;186:105349. doi: 10.1016/j.biocontrol.2023.105349</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Государственный реестр селекционных достижений, допущенных к использованию. Т. 1. Сорта растений. [Электронный ресурс]. URL: https://gossortrf.ru/registry/ (дата обращения: 10. 04. 2025)</mixed-citation><mixed-citation xml:lang="en">State Register for Selection Achievements Admitted for Usage (National List). Vol. 1 “Plant varieties.” (In Russ.) URL: https://gossortrf.ru/registry/ (accessed: April 10, 2025).</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Методические указания по регистрационным испытаниям фунгицидов в сельском хозяйстве / Под ред. В.И. Долженко. Санкт-Петербург: Всероссийский научно-исследовательский институт защиты растений РАСХН, 2009. 378 с. EDN: WERHOZ</mixed-citation><mixed-citation xml:lang="en">Methodological guidelines for registration tests of fungicides in agriculture. V.I. Dolzhenko (Ed). St. Petersburg, Russia: All-Russian Research Institute of Plant Protection, 2009:378. (In Russ.) EDN: WERHOZ</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
